橘小实蝇和番石榴实蝇对杧果挥发物的电生理反应

Electrophysiological Responses of Bactrocera dorsalis and Bactrocera correcta to Volatiles from Mango Fruits

【目的】探究橘小实蝇(Bactrocera dorsalis)和番石榴实蝇(Bactrocera correcta)与寄主植物间化学通讯的差异性,以助于阐明两者的同域发生与分布,为开发植物源诱剂提供科学依据。【方法】采用触角电位EAG技术测定了橘小实蝇、番石榴实蝇对杧果挥发物的嗅觉反应。【结果】橘小实蝇、番石榴实蝇均对杧果挥发物产生触角电位反应,其触角电位反应值均显著高于对照液状石蜡的反应值(P<0.05),且雄虫、交配和未交配雌虫对植物气味化合物产生了不同的触角电位反应,对反-2-己烯醛的EAG反应值最高,而对3-蒈烯、异松油烯、ME的EAG反应相对值较低;橘小实蝇和番石榴实蝇对同一挥发物的电生理反应也存在差异。【结论】橘小实蝇、番石榴实蝇与寄主植物间的化学通讯具有差异性;3-蒈烯可能成为番石榴实蝇潜在性的两性诱剂。

[Purpose]In order to help explain infection and distribution of oriental fruit fly,B.dorsalis and guava fruit fly,B.correcta in same area,and to provide a theoretical basis for the development of attractants,the differences of chemical communication between the two species and host plant were explored,respectively.[Method]Olfactory response of the oriental fruit fly and guava fruit fly to volatiles from mango fruits was determined by EAG.[Result]Volatiles from mango fruits elicited EAG responses of both oriental fruit fly and guava fruit fly,which showed a significantly higher EAG response than the control(liquid paraffin)(P<0.05),and different EAG responses of males,mated and unmated females to mango odors were recorded,among which the EAG value to(E)-2-hexenal was the highest,with relatively low values to 3-carene,α-terpinolene,methyl eugenol.Oriental fruit fly presented different EAG responses from guava fruit fly to the same compound due to interspecific difference and the long-term adaptation of oriental fruit fly,guava fruit fly to host odors.[Conclusion]The chemical communication between the two and the same host plant was very different,and3-carene had a potential to become lures for both sexes of guava fruit fly.