市售鸡蛋壳表面携带β-内酰胺酶耐药基因假单胞菌的分离鉴定

Isolation and Identification of Pseudomonas Carrying β-lactamase Resistant Gene on the Surface of Egg Shell

为研究鸡蛋壳表面携带耐药细菌的情况,选取吉林省四家蛋鸡养殖场内新鲜鸡蛋,采取传统细菌学方法从鸡蛋壳表面进行病原菌分离鉴定和药敏试验,PCR方法进行耐药基因检测,成功分离鉴定出4株嗜麦芽窄食假单胞菌;抑菌试验结果显示,分离菌对环丙沙星敏感,对林可霉素、氟苯尼考、阿米卡星中敏感,对头孢曲松、阿莫西林、多粘菌素E不敏感;在4株假单胞菌分离株中检出CTX-M型β-内酰胺酶耐药基因。此结果为进一步研究假单胞菌对蛋鸡养殖的危害和食品卫生安全提供了试验依据。

In order to study the drug-resistant bacteria on the surface of egg shell,fresh eggs were selected from four chicken farms in Jilin province,the traditional bacteriological method was used to identify the pathogen and drug susceptibility test,and the PCR method was used to detect the drug resistance gene from egg shell surface.The results showed that: four strains of Pseudomonas maltophilia were successfully isolated and identified. The results of the antibiotic resistance test showed the isolate was highly sensitive to ciprofloxacin; was insensitive to ceftriaxone,amoxicillin,polymyxin,and it had different degrees to other drugs. The CTX-M type β-lactamase resistance genes were detected in Pseudomonas isolates. This provides an experimental basis for further study on the harm of Pseudomonas in layer culture and food hygiene safety.