市售小麦面粉中过氧化苯甲酰的检测

Detection of Benzoyl Peroxide in Wheat Flour on Sale

采用分光光度法构建面粉中过氧化苯甲酰的快速检测体系。采用单因素实验设计优化过氧化苯甲酰的检测体系,建立工作曲线,并构建出面粉中的过氧化苯甲酰检测模型。研究结果表明,最佳检测体系为在检测波长510nm条件下,pH为4.4,检测温度为50℃,反应时间为2min;在0.001～0.01mg/mL范围内,BPO浓度与吸光度呈良好线性关系,两种面粉的工作曲线分别为y=-3.228 9x+0.557 4和y=-8.328 3x+0.587 7,R2分别为0.983 2和0.982 4,方法的最低检出限为0.65 mg/kg和0.58 mg/kg,相对标准偏差RSD均小于5%。该法用于两份市售面粉中过氧化苯甲酞的测定,平均回收率分别为100.82%和99.11%,所建立的方法方便、快捷且灵敏度较高。

In this paper,the rapid determination of trace amounts of benzoyl peroxide(BPO)in wheat flour was performed by spectrophotometry.The optimization of BPO detection system with the single factor method was established by standard curves.Finally,we get the corresponding parameter of two commercial samples.The best system was selected:pH was 4.4,temperature was 50℃and the reaction time was 2 min unber the 510 nm wavelength.The results show that the absorbance showed a good linear relationship with BPO concentration within the range of 0.001~0.01mg/L.The working curves of the two kinds of commercial flour samples were y=-3.2289x+0.5574and y=-8.3283x+0.5877,respectively.R2 were 0.9832 and 0.9824 respectively.The lowest detection limits of the two commercial flour samples were 0.65mg/mL and 0.58mg/mL,respectively.The relative standard deviations(RSD)were all less than5%.The method developed is convenient and fast and has a relatively high sensitivity and the detection results are satisfactory.The average spike recovery rates of BPO in the two commercial flour samples were 100.82% and 99.11%,respectively.