酶法提取杏仁粕中残油的研究

The Research on Enzymatic Extraction of Reliquus Oil from Almond Residue

采用酶法提取杏仁粕中残油,通过单因素试验确定原料粒度为100目,提取酶为中性蛋白酶和纤维素酶的混合酶1∶1(质量比)。选取酶添加量、酶解p H值、酶解时间、酶解温度4个主要因素,进行L(349)正交优化试验。通过试验得出最佳提取条件为:酶的添加量为3%,酶解pH值为7.5,酶解温度为40℃,酶解时间为2.5 h,出油率为26.25%。得到呈棕黄色,透明,具有浓郁杏仁香味的油脂。

This paper adopted enzymolysis to extract can oil from almond residue,through the single element experiment,we definited that the material's granularity was 100,the mixed-enzymes of neutral protease and cellulase(1∶1)was the extracted enzyme.The amount of enzyme,the enzymolysis pH,the enzymolysis time and the enzymolysis temperature were chosen as the main factors based on the single element and L9(34)orthogonal experiment were optimized.The best condition for extraction by the orthogonal experiment was summarized:the amount of enzyme was 3%,the enzymolysis pH was 7.5,the enzymolysis temperature was 40℃,the enzymolysis time was 2.5 h,the oil yield was 26.25%.The quality of the reliquus oil was yellow,diaphanous and had strong almond flavor.