苍术炒焦前后正丁醇部位对湿阻中焦证大鼠水盐代谢影响

Effects of n-butyl Alcohol Parts Extracted from Atractylodis Lancea Before and After Deep-Fired on Water and Salt Metabolism in Rats with Syndrome of Accumulation of Dampness in Middle-Jiao

目的:通过研究苍术炒焦前后正丁醇部位对湿阻中焦证大鼠水盐代谢的药效,阐释焦苍术药效改变的机制,为临床合理用药提供客观依据。方法:以湿阻中焦证大鼠为模型,比较不同给药组大鼠血清醛固酮(ALD)、肾小管水孔蛋白2(AQP2)和血浆K^+、Na^+、Cl^-等电解质水平。结果:模型组大鼠血清ALD、肾小管AQP2与正常组相比明显升高,血浆K^+、Na^+和Cl^-浓度均有所降低,以K^+浓度降低较为显著;与模型组比较,各给药组均表现为ALD和AQP2不同程度降低,其中生品正丁醇部位高剂量组(正生高)对ALD和AQP2水平有显著性影响,且其调节血浆电解质水平能力与阳性组无显著性差异(P<0.05)。结论:苍术炒焦以后,燥性得以缓和,焦苍术对模型大鼠水盐代谢的调节作用明显减弱。

Objective:Through the study of n-butanol part extracted from Rhizoma Atractylodis before and after the deep-fired on the water and salt metabolism in rats with syndrome of accumulation of dampness in middle-Jiao,to interpret the mechanism of efficacy change for the focal Rhizoma Atractylodis and provide objective basis for clinical useing drugs reasonably.Methods:Syndrome of Accumulation of Dampness in Middle-Jiao model rats were established to compare each group's different drug treatment on serum aldosterone(ALD),renal tubular hole protein 2(AQP2)and serum K+,Na+,Cl-electrolyte levels.Results:Compared with normal group,serum ALD and renal tubular AQP2 increased significantly in the model group.At the same time,the concentrations of K+,Na+and Cl– in plasma were all reduced,but the K+concentration reduced considerably.Compared with the model group,the levels of ALD and AQP2 were reduced in each group for different drug treatments.Particularly,the high dose group's extract from raw product had significant difference compared with the model group on impacting on the ALD and AQP2 levels.There was no significant difference in plasma electrolyte levels to the positive group also(P<0.05).Conclusion:After deep-fired,the dry temper of Rhizoma Atractylodis has been eased and its adjusting ability reduced significantly on the water and salt metabolism in rats with syndrome of accumulation of dampness in middle-Jiao.