HPLC同时测定聚醚多元醇中的小分子醛和丙酮

Simultaneous Determination of Aldehydes and Acetone in Polyether Polyols by HPLC

用2,4-二硝基苯肼进行柱前衍生,建立了高效液相色谱同时测定聚醚多元醇中甲醛、乙醛、丙烯醛、丙酮和丙醛的方法,考察了衍生反应时间、温度及衍生试剂体积对反应的影响。结果表明,该方法检出限为0. 015～0. 25 mg/kg,线性相关系数R≥0. 9998。样品测定精密度为1. 03%～2. 75%,加标回收率为91. 0%～106. 7%。该方法已成功地用于实际样品检测,解决了聚醚多元醇中丙烯醛和丙酮分离的技术难题,具有线性范围宽,精密度好,回收率高等优点。

A high performance liquid chromatography(HPLC)method based on the 2,4-dinitrophenylhydrazine(DNPH)as pre-derivatization agent was developed for the determination of formaldehyde,acetaldehyde,acraldehyde,acetone and propionaldehyde.The influences of derivatization time,temperature and reagent volume were tested.Results indicated that the range of detection limit was 0.015~0.25 mg/kg,the correlative coefficients of linear regression function for aldehydes and acetone were all higher than 0.9998.Furthermore,the relative standard deviation for determination of samples was 1.03%~2.75% and the recovery rate was 91.0%~106.7%.Finally,the proposed method with wide measurement range,good precision and high recovery rate,was successfully applied to the analysis of polyether polyol samples,and it solved the separation problem of acraldehyde and acetone.