植物线粒体巯基亚硝基化修饰蛋白质组学研究进展

Advances on plant mitochondrial S-nitrosylation proteomics

蛋白质S-亚硝基化是一氧化氮(NO)与蛋白质半胱氨酸残基(Cys)共价连接形成S-亚硝基硫醇(-SNO)的过程,被认为是植物中体现NO生物活性的最重要途径.线粒体在依赖S-亚硝基化的NO信号转导中起关键作用.综述了应用蛋白质组学技术鉴定的植物线粒体S-亚硝基化蛋白质的特征,为认识线粒体NO调控网络体系中重要的信号与代谢通路(如光呼吸、三羧酸循环、氧化磷酸化、活性氧分子(ROS)稳态,以及蛋白质加工与周转)提供了线索.

Protein S-nitrosylation is the process in which nitric oxide (NO) moiety is covalently attached to protein cysteine (Cys) to form S-nitrosothiol (-SNO).It is considered to be the most important way to reflect the biological activity of NO in plants.Mitochondria play a key role in NO signaling dependent on S-nitrosylation.Proteomics approach has proven to be a very efficient tool for identifying S-nitrosylated proteins in plants.The findings provide insight into important signaling and metabolic pathways in the NO regulatory network of mitochondria (e.g.,photorespiration,tricarboxylic acid cycle,oxidative phosphorylation,reactive oxygen species (ROS)homeostasis,and protein processing and turnover).