摘要
石油醚、乙酸乙酯作为萃取剂,利用聚酰胺凝胶柱分离,乙醇/水梯度洗脱以分离丹参(Salvia miltiorrhiza),得到丹参酮ⅡA.所得到的产物经过核磁共振谱(1H NMR、13C NMR)确证了结构,并经薄层色谱(TLC)、高效液相色谱(HPLC)分析,显示其纯度达到93%以上.本研究提供了一种高效的丹参酮ⅡA的提取分离方法,具有提取率高、成本低、安全可靠等优点,也为其他药用植物有效成分的分离提供参考.
In this study,petroleum ether and ethyl acetate were used as extraction agents,and polyamide column chromatography was used to separate tanshinone ⅡA using ethanol/water as eluting solvents.TLC, 1H NMR, 13 C NMR,HPLC analysis revealed that the purity of the extracted tanshinone ⅡA was over 93%.Above all,this study provides a highly efficient extraction and separation method for tanshinone ⅡA which displayed a series of advantages including higher yield,lower cost,higher safety and reliability,etc.And it also provides a new approach for purification of other active ingredients from medicinal plants.
作者
张毅
邓昌平
时敏
付蓉
王瑶
曹建国
开国银
ZHANG Yi;DENG Changping;SHI Min;FU Rong;WANG Yao;CAO Jianguo;KAI Guoyin(Development Center of Plant Germplasm Resources,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;College of Pharmacy,Zhejiang Chinese Medical University,Hangzhou 311402,Zhejiang,China)
出处
《上海师范大学学报（自然科学版）》
2018年第6期754-761,共8页
Journal of Shanghai Normal University(Natural Sciences)
基金
国家自然科学基金(81522049,31270007,31571735,30900110)
上海市科学技术委员会项目(17JC1404300,15430502700)
上海市曙光学者计划(16SG38)
浙江省高校高水平创新团队资助
上海市协同创新中心项目(ZF1205)
上海植物种质资源工程技术研究中心项目(17DZ2252700)
