盐胁迫下不同品种水稻的基因表达

Gene Expression of Different Rice Varieties under Salt Stress

[目的]初步探索水稻耐盐机制,筛选关键基因。[方法]从公共基因芯片数据库GEO(Gene Expression Omnibus)搜索有关盐胁迫下水稻基因表达的数据,利用TAC(Transcriptome Analysis Console)筛选表达差异基因,对差异基因进行GO(Gene Ontology)富集分析、信号通路富集分析。[结果]在盐胁迫下,CSR11、PL177两种耐受型水稻与IR64、VSR156两种敏感型水稻分别有3 289、1 934、1 806、4 426个基因表达。耐受型水稻中同时上调或下调的基因有288个,敏感型水稻中同时上调或下调的基因有330个。通过生物信息学研究发现,耐受型水稻盐胁迫主要影响代谢途径、次级代谢产物合成、转录因子等方面。敏感型水稻盐胁迫影响主要影响胁迫响应、激素转导、物质代谢等方面。[结论]利用生物信息学方法能有效筛选目标基因,并可为进一步研究提供依据。

[Objective] To explore the mechanism of the salt tolerance and screen the key genes of salt tolerance gene in rice. [Method]The dates of whole genomic expression profiles got from the salt tolerant rice and salt sensitive rice were obtained from GEO database and identified differential expressed genes by TAC software. The differential gene expression were characterized by gene ontology( GO),KEGG pathway. [Result] For salt tolerant rice( CSR11,PL177),there were 3 289,1 934 differential expression genes under salt stess. For salt sensitive rice( IR64,VSR156),there were 3 289,1 934 different expression genes under salt stess. There were 288 genes that were simultaneou up-regulated or down-regulated in tolerant rice. There were 330 genes that were simultaneous up-regulated or down-regulated in sensitive rice. An analysis of differentially expressed gene pathways revealed that metabolic pathways,secondary metabolites synthesis,transcription factors in tolerant rice,but stress response,hormone transduction and material metabolism in sensitive rice. [Conclusion] The key genes could be screened by bioinformatics effectively,which might provide the basis for further experimental study.