香芹茎叶挥发油提取工艺及清除DPPH自由基活性研究

Study on Extraction Process of Volatile Oil from Stem and Leave of Libanotis seseloides and Its Activity of Scavenging DPPH Free Radical

[目的]研究水蒸气蒸馏法提取香芹茎、叶挥发油的工艺优化,并测定其抗氧化活性。[方法]采用单因素试验和响应面法优化考察浸泡时间、料液比和蒸馏时间对挥发油得率的影响,并以清除DPPH自由基能力评价香芹茎、叶挥发油的抗氧化活性。[结果]香芹茎、叶挥发油的最佳提取条件分别为:浸泡时间1.5 h、料液比1∶6 (g∶mL)、蒸馏时间3.0 h和浸泡时间2.0 h、料液比1∶8、蒸馏时间3.0 h,此时香芹茎和叶挥发油提取率分别为0.917%和1.284%。香芹茎、叶挥发油均具有一定的清除DPPH自由基能力,香芹叶挥发油清除DPPH自由基能力比香芹茎强。[结论]该研究为进一步开发香芹药食两用价值提供理论依据。

[Objective]The research aimed to study the process optimization of extracting volatile oil from Libanotis seseloides stem and leaf by steam distillation and determine its antioxidant activity.[Method]The effects of soaking time,solid-liquid ratio and distillation time on the extraction of volatile oil were studied by single factor and response surface methodology.The antioxidant capacity of volatile oil from its stems and leaf was evaluated by scavenge rate against DPPH free radical.[Result] The optimum extraction conditions for the volatile oil of Libanotis seseloides stem and leaf were as follows: soaking time 1.5 h,solid-liquid ratio 1 ∶6( g ∶mL),distillation time 3.0 h,and soaking time 2 h,solid-liquid ratio 1 ∶8,distillation time 3.0 h.The extraction rates of volatile oil from Libanotis seseloides leaf were 0.917% and 1.284%,respectively.The volatile oil of the stems and leaf had certain scavenging ability to DPPH free radical,and the volatile oil of Libanotis seseloides leaf had better scavenging ability on DPPH free radicals than its stems.[Conclusion]This study provides a theoretical basis for further development of the dual-use value of Libanotis seseloides.