氧化石墨烯固定化α-淀粉酶

Immobilized α-amylase ongraphene oxide

以石墨粉为原料,使用改进Hummer法制备氧化石墨烯,采用沉积法固定化α-淀粉酶,并对固定条件进行优化。分别采用NaOH-CH_2ClCOOH法和HNO_3-H_2SO_4法制备羧基化氧化石墨烯,并将其用于固定化α-淀粉酶。结果表明,沉积法固定化酶的最适宜温度为65℃,最适宜pH=7. 0。连续催化反应9次后,固定化酶活力仍能保持初始固定化酶活力的47. 81%。经比较发现,NaOH-CH_2ClCOOH法更有利于羧基化氧化石墨烯的制备,该方法制备的羧基化氧化石墨烯产率为HNO_3-H_2SO_4法的1. 2倍。

Graphite powder was used as raw material to prepare graphene oxide by improved Hummer method,and α-amylase was immobilized by deposition method and the immobilization conditions were optimized. The carboxylated graphene oxide was prepared by NaOH-CH2ClCOOH and HNO3-H2SO4,respectively,and used for immobilizing of α-amylase. Results showed that the optimum temperature for immobilization of the enzyme was 65 ℃ and the optimum pH was 7. 0. After 9 consecutive using,activity for enzyme immobilization remained 47. 81% of the initial activity. It was found that NaOH-CH2Cl COOH was more conducive for preparation of carboxylated graphene oxide. Yield of carboxylated graphene oxide prepared by NaOH-CH2ClCOOH was 1. 2 times than by HNO3-H2SO4.