腹内压改变对慢性腹水模型大鼠肾素-血管紧张素系统及葡萄糖代谢的影响

Effect of intra-abdominal pressure on renin-angiotensin system and glucose metabolism in rat model of chronic ascites

目的:探讨腹内压力对高血压相关指标肾素、血管紧张素Ⅰ(AngⅠ)、血管紧张素Ⅱ(AngⅡ)和葡萄糖代谢相关指标空腹血糖、糖耐量、胰岛素的影响。方法:将19只大鼠随机分成生理盐水组(对照组)和矿物油组(实验组),实验组腹腔连续间断注射矿物油建立慢性腹水大鼠模型,对照组连续间断注射相同量的生理盐水,5周后测量腹内压,检测肾素、AngⅠ、AngⅡ、胰岛素、空腹血糖水平和糖耐量功能。结果:与对照组相比,实验组肾素与AngⅠ水平明显降低(P<0.05或P<0.01),胰岛素及AngⅡ比较,差异无统计学意义,空腹血糖水平明显降低(P<0.01),葡萄糖激发后的30 min及1 h血糖水平明显升高(P<0.05或P<0.01),2 h血糖水平无显著差异。结论:单独增加腹内压力可能在该动物模型中引起肾素-血管紧张素系统紊乱和葡萄糖代谢异常。

Objective: Overweight and obesity, especially those with abdominal obesity, are more likely to have metabolic diseases. Studies have also shown that pregnant women are more prone to metabolic diseases than non-pregnant women. To investigate the relationship between metabolic diseases and increase in intra-abdominal pressure (IAP), this study investigated the effects of increased IAP on fasting blood glucose, glucose tolerance, and insulin in glucose metabolism and the indicators of hypertension, such as renin, angiotensin Ⅰ( Ang Ⅰ),angiotensin Ⅱ( Ang Ⅱ). Methods : Nineteen rats were randomly divided into normal saline group and mineral oil group. The experimental group was treated with intermittent intraperitoneal injection of mineral oil to establish a chronic ascites rat model. The control group was continuously injected with the same amount of saline continuously. The intra-abdominal pressure renin, angiotensin Ⅰ, angiotensin Ⅱ, insulin, fasting glucose levels, glucose levels at half an hour and glucose at 1 and 2 hours after glucose challenge were measured after 5 weeks. Results : Compared with the control group, the levels of renin and angiotensin Ⅰ were significantly lower in the experimental group (P<0.05 or P<0.01). There was no significant difference in insulin and angiotensinⅡ between the two groups, and the fasting blood glucose level was significantly reduced (P<0.01). Glucose levels were significantly increased at half an hour and Ⅰ hour after glucose challenge (P<0.05 or P<0.01), and there was no significant difference in blood glucose level at 2 hours. Conclusion: Increasing intra-abdominal pressure alone may cause renin-angiotensin system disorders and abnormal glucose metabolism in this animal model.