妊娠期糖尿病孕妇血清miR-126、sVCAM-1水平与糖脂代谢的相关性

Correlation between serum miR-126 expression and sVCAM-1 level and glycolipid metabolism of pregnant women with gestational diabetes mellitus

目的:探讨妊娠期糖尿病(GDM)孕妇血清miR-126、血管内皮细胞黏附分子-1(sVCAM-1)表达特点及与糖脂代谢关系。方法:连续性选取2016年2月-2018年2月于本院就医的GDM患者85例为研究对象(观察组),并选择同期本院正常妊娠产检孕妇60例为对照组,检测两组血清miR-126、sVCAM-1水平,空腹血糖(FPG)、空腹胰岛素(FINS),胰岛素抵抗指数(HOMA-IR)、三酰甘油(TG)、胆固醇(TC)、载脂蛋白A(apoA)、载脂蛋白B(apoB)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),分析miR-126、sVCAM-1与糖脂代谢异常的相关性。结果:观察组血清TG、LDL-C、apoA、apoB水平高于对照组,HDL-C低于对照组(均P<0.05),TC水平两组对比无差异(P>0.05)。观察组FPG、FINS、HOMA-IR、sVCAM-1均高于对照组,miR-126表达低于对照组(均P<0.05)。miR-126与TG、LDL-C、FPG、FINS、HOMA-IR、apoA呈负相关(r=-0.328、-0.510、-0.324、-0.336、-0.616、-0.456、-0.315,P<0.05),与HDL-C呈正相关(r=0.335,P<0.05),与TC、apoB无关(r=-0.013、-0.015,P>0.05)。sVCAM-1与TG、FPG、HOMA-IR呈正相关(r=0.433、0.637、0.301,P<0.05),与HDL-C呈负相关(r=-0.325,P<0.05),与TC、LDLC、FINS、apoA、apoB无关(r=0.040、0.158、0.012、0.008,P>0.05)。TG(OR=2.310,95%CI 0.681～1.264)、HOMA?IR(OR=1.272,95%CI 0.261～0.961)是影响miR-126表达的独立因素,TG(OR=1.862,95%CI 0.136～0.769)与sVCAM-1水平独立相关。结论:GDM患者血清miR-126表达下调,sVCAM-1水平升高,血清miR-126、sVCAM-1可能参与GDM患者糖脂代谢异常过程。

Objective:To investigate the expression of serum micro RNA-126 and level of vascular endothelial cell adhesion molecule-1(sVCAM-1)of pregnant women with gestational diabetes mellitus(GDM),and to analyze their relationship with glycolipid metabolism of pregnant women with GDM.Methods:85 pregnant women with GDM from February 2016 to February 2018 were selected in study group,and 60 normal pregnant women were selected in control group.The miR-126 expression,and serum levels of sVCAM-1,fasting blood glucose(FPG),fasting insulin(FINS),glycerol(TG),cholesterol(TC),apolipoprotein A(apoA),apolipoprotein B(apoB),high-density lipoprotein cholesterol(HDL-C),and low density lipoprotein cholesterol(LDL-C)of all pregnant women were detected,and insulin resistance index(HOMA-IR)was calculated.The correlation between miR-12 expression,sVCAM-1 level and indexes of abnormal glycolipid metabolism was analyzed.Results:The serum levels of TG,LDL-C,apoA and apoB of pregnant women in study group were significant higher than those of pregnant women in control group(P<0.05),and the HDL?C level of pregnant women in study group was significant lower than that of pregnant women in control group(P<0.05),but there was no significant different in TC level between the two groups(P>0.05).The levels of FPG,FINS,HOMA-IR and sVCAM-1 of pregnant women in study group were significant higher than those of pregnant women in control group(P<0.05),and the expression of miR-126 of pregnant women in study group was significant lower than that of pregnant women in control group(P<0.05).The expression of miR-126 was negatively correlated with the level of TG,LDL-C,FPG,FINS,HOMA-IR or apoA(r=-0.328,-0.510,-0.324,-0.336,-0.616,-0.456,-0.315,P<0.05),and the expression of miR-126 was positively correlated with the level of HDL-C(r=0.335,P<0.05),but the expression of miR-126 was irrelevant with the level of TC or apoB(r=-0.013,-0.015,P>0.05).sVCAM-1 level was positively correlated with the level of TG,FPG or HOMA-IR(r=0.433,0.637,0.301,P<0.05),sVCAM-1 level was negatively correlated with the level of HDL-C(r=-0.325,P<0.05),sVCAM-1 level was irrelevant with the level of TC,LDL-C,FINS,apoA,or apoB(r=0.040,0.158,0.012,0.008,P>0.05).The level of TG(OR=2.310,95%CI:0.681-1.264)or HOMA-IR(OR=1.272,95% CI:0.261-0.961)was the independent influencing factor for the expression of miR-126,and the level of TG(OR=1.862,95 %CI:0.136-0.769)was the independent influencing factor for sVCAM-1 level.Conclusion:Serum miR-126 expression of pregnant women with GDM is significantly down-regulated,and sVCAM-1 level of pregnant women with GDM is increased.Serum miR-126 and Serum sVCAM-1 may be involved in abnormal glycolipid glucose metabolism of pregnant women with GDM.