摘要
利用PKS-NRPS基因筛选与抗菌活性测定,选取筛选结果为阳性以及广谱抗菌的FIM060022菌株进行多相分类鉴定。结果显示,该菌株对大肠埃希菌(E. coli)、金黄色葡萄球菌(S. aureus)、枯草芽胞杆菌等细菌(B. subtilis),以及白色念珠菌(C. albicans)、红酵母(R. rula)与新型隐球菌(C. neoformans)等真菌具有抗菌活性。通过对菌株的培养特征、形态观察、生化特性、细胞壁组成分析、脂肪酸组成分析、G+C mol%含量组成,结合16S r DNA序列以及PKS/NRPS基因序列分析,将菌株FIM060022鉴定为小单胞菌科疣孢菌属。结果提示FIM060022是一株具有广谱抗菌活性的海洋稀有放线菌,具有产药用化合物的潜能。
Using PKS-NRPS gene screening and antibacterial activities determination, a strain FIM060022 that had positive result and broad spectrum in antibiotics was selected and carried out polyphasic classification characterization. The results showed the strain possessed antibacterial activities against E. coli, Staphylococcus aureus, Bacillus subtilis, Candida albicans, Rhodotorula, Cryptococcus neoformans, and other fungi. The cultivation characteristic, morphology observation, biochemical properites, analysis of its cellwall components, analysis of fatic acids components, G+C mol % content component, combined with 16S rDNA sequence as well as PKS/NRPS gene sequence analysis, the strain FIM060022 characterized as Micromonosporaceae and genus Verrucosispora. And the results pointed out that the strain FIM060022 was a rare marine Actinomyces strain possessing antibacterial activities of broad spectrum, and possessed a potential ability to produce medicine chemical compound.
作者
赵薇
谢阳
陈路劼
连云阳
江红
ZHAO Wei;XIE Yang;CHEN Lu-jie;LIAN Yun-yang;JIANG Hong(Fujian Prov. Key Lab. of Screen. for Novel Microb. Prod., Fujian Inst. of Microbiol., Fuzhou 350007)
出处
《微生物学杂志》
CAS
CSCD
2018年第6期44-49,共6页
Journal of Microbiology
基金
国家科技重大专项(2017zx09101003-007-007)
福建省公益类项目(2015R1009-6
2016R1009-1)
福建省自然基金项目(2016J01345)
福建省海洋高新项目(闽海洋高新[2016]25号)
关键词
基因筛选
抗菌活性筛选
疣孢菌
多相分类鉴定
gene screening
antibacterial activities screening
Verrucosispora
polyphasic taxonomy characterization