桔梗皂苷D对小鼠淋巴细胞和巨噬细胞免疫功能的影响

Effect of platycodin D on lymphocyte and macrophage immune function of mice

【目的】探讨桔梗皂苷D对小鼠淋巴细胞和巨噬细胞免疫功能的影响。【方法】无菌分离小鼠脾淋巴细胞和腹腔巨噬细胞,制备淋巴细胞和巨噬细胞悬液。分别培养这2类细胞,并分为空白对照组、阳性对照组(左旋咪唑处理细胞)及25,50,75和100μg/mL桔梗皂苷D组,采用MTT法检测桔梗皂苷D对淋巴细胞增殖和巨噬细胞吞噬功能的影响,采用ELISA法检测桔梗皂苷D对淋巴细胞IL-2、IL-4和巨噬细胞TNF-α、IL-12分泌的影响。【结果】桔梗皂苷D能够促进淋巴细胞增殖,增强巨噬细胞的吞噬功能,并可刺激淋巴细胞IL-2、IL-4和巨噬细胞TNF-α、IL-12的分泌,其中以50μg/mL组效果最佳。【结论】桔梗皂苷D能增强小鼠淋巴细胞和巨噬细胞的免疫调节活性。

【Objective】The study investigated the effects of platycodin D on immune function of lymphocyte and macrophage of mouse.【Method】The lymphocytes and macrophage suspensions were prepared through sterile isolation of mouse spleen lymphocytes and peritoneal macrophages.Two types of cells were cultured separately with blank control group,positive control group(cells were treated by levamisole),and four experimental groups with platycodin D concentrations of 25,50,75 and 100μg/mL.The effects of platycodin D on lymphocyte proliferation and macrophage phagocytosis were detected by MTT assay.The effects of platycodin D on secretion of IL-2 and IL-4 in lymphocyte and secretion of TNF-αand IL-12 in macrophages were detected by ELISA.【Result】Platycodin D promoted lymphocyte proliferation,enhanced phagocytosis of macrophage,and stimulated secretion of IL-2 and IL-4 in lymphocyte and secretion of TNF-αand IL-12 in macrophages.The concentration of 50μg/mL was the best.【Conclusion】Platycodin D enhanced the immunomodulatory activities of mouse lymphocytes and macrophages.