三种白术提取物对雏鸡新城疫抗体水平的影响

The Effect of Three Kinds of Extract of Atractylodes Macrocephala on Newcastle Disease Antibody in Chickens

为了探讨白术对雏鸡体液免疫功能的影响,以黄芪多糖为阳性对照,采用Elisa法分别检测了三种白术提取物在连续给药8 d和15 d后对雏鸡新城疫抗体水平的影响。结果表明,连续给药8 d后,与空白组相比,各组差异均不显著,其中高剂量白术醇提物和黄芪多糖分别使新城疫抗体水平提高了15.35%(P>0.05)和15.32%(P>0.05)。连续给药15 d后,与空白组相比,各组抗体水平均有不同程度的升高,其中高剂量白术水提物和低剂量白术醇提物分别使抗体水平升高了61.37%(P<0.05)和32.57%(P<0.05);与阳性组相比,高剂量白术水提物使抗体水平显著提高了45.02%(P<0.05)。高剂量白术水提物连续给药15 d对雏鸡体液免疫增强效果最好。

In order to explore the effect of Atractylodes macrocephala(AM)on humoral immunity in chickens,three kinds of extract of AM were prepared.Astragalus polysaccharides(APS)was used for positive control,the different extracts were added into the feedstuff of chickens.The levels of Newcastle disease antibody in chickens of each group were respectively detected by ELISA after 8 days and 15 days continuous administration.The results showed that,after continuous administration of 8 days,compared with the blank group,the differences among groups were not significant.The high dose of AM ethanol extract and APS increased the antibody level of Newcastle disease by 15.35%(P>0.05)and 15.32%(P>0.05)respectively.After continuous administration of 15 days,compared with the blank group,the level of antibody in each group increased in varying degrees.High dose AM aqueous extract and low dose AM ethanol extract increased the antibody level by 61.37%(P<0.05)and 32.57%(P<0.05)respectively.Compared with the positive group,the high dose of AM aqueous extract increased the antibody level by 45.02%(P<0.05).The high dose of AM aqueous extract was continuous administrated for 15d had the best effect on enhancing the humoral immunity of chickens.