甲基丙二酸质控滤纸片的配制及其气相色谱-质谱联用技术检测尿液有机酸的应用评估

Preparation of methylmalonic acid control filter paper and application evaluation in the determination of urinary organic acid by gas chromatography-mass spectrometry

目的探讨甲基丙二酸质控滤纸片的配制方法及其在气相色谱-质谱联用技术测定尿有机酸方法中的应用价值。方法收集健康者尿液标本,根据肌酐值及目标浓度添加甲基丙二酸溶液,配成甲基丙二酸尿液。采用4cm×6cm专用滤纸片浸取甲基丙二酸尿液后自然晾干,制成甲基丙二酸质控滤纸片。根据CNAS-GL03要求,对质控滤纸片的均匀性、稳定性进行评价。结果甲基丙二酸质控滤纸片的最终浓度达到目标浓度,均匀性、稳定性评估结果表明该质控品基质均匀、稳定期至少1年。结论本研究成功配制了用于气相色谱-质谱联用技术检测有机酸的甲基丙二酸质控滤纸片,为研制含其他有机酸的复合项目室内质控品提供了思路。

Objective To explore the method of preparing methylmalonic acid control based filter paper in laboratoty and its value in gas chromatography-mass spectrometry for the determination of urinary organic acid.Methods Normal urine samples were collected,and methylmalonic acid solution was added according to the creatinine value and the target concentration,dubbed methylmalonic acid urine.With 4 cm×6 cm special filter paper leaching methylmalonic acid urine and then dried to produce methylmalonic acid quality control filter paper,uniformity and stabilityand was evaluated according to CNAS-GL03.Results The final concentration of methylmalonic acid control filter paper reached the target concentration,and the uniformity and stability results showed that the quality control matrix was uniform and stable for at least one year.Conclusion The methylmalonic acid control filter paper has been successfully prepared for the detection of organic acids by gas chromatography-mass spectrometry.It fills the blank of the test system without indoor quality assessment,and provides ideas for the development of composite project with other organic acids.