三七总皂苷预处理对大鼠心肌缺血/再灌注损伤的影响

Sanchi Total Saponins Pretreatment Protects Rats with Myocardial Ischemia/Reperfusion Injury

目的:观察三七总皂苷预处理对大鼠心肌缺血/再灌注损伤(MI/RI)的作用。方法:通过结扎冠脉30 min再灌注2 h的方式制备MI/RI模型,将大鼠随机分为4组(每组10只):假手术组、模型组、三七总皂苷高剂量组(60 mg/kg)、三七总皂苷低剂量组(30 mg/kg),于制备模型前7 d开始腹腔注射给药;再灌注结束后,采用比色法检测血清肌酸激酶(CK)活力和乳酸脱氢酶(LDH)活力,染色法检测心肌梗死面积(MIA)。结果:与模型组比较,三七总皂苷高、低剂量组大鼠MIA均有显著缩小(P<0.05);与模型组比较,三七总皂苷高、低剂量组血清CK活力均有显著降低(P<0.05或P<0.01),LDH活力亦有明显降低(P<0.01)。结论:三七总皂苷预处理可保护MI/RI心肌受损,其作用途径可能与改善心肌细胞膜的稳定性相关。

Objective:To observe the effect of panax notoginseng saponins preconditioning on myocardial ischemia/reperfusion injury(MI/RI)in rats.Method:The MI/RI model was established by ligating coronary artery for 30 min and reperfusion for 2 h.The rats were randomly divided into 4 groups(10 rats in each group):Sham operation group,model group,Panax notoginseng saponins high dose group(60 mg/kg),panax notoginseng saponin low dose group(30 mg/kg).Before the modeling operation,STS was injected intraperitoneal once daily for 7 days.After the modeling operation,creatine kinase and lactate dehydrogenase activities in sera of rats were detected by chromatometry,myocardial infarction size(MIA)in rats were examined by staining method.Result:Compared with the model group,the total saponins of Panax notoginseng decreased significantly in both groups(P<0.05).Compared with the model group,the total saponins of Panax notoginseng were higher than those of the control group.The serum CK activity in the low dose group was significantly lower than that in the model group(P<0.05 or P<0.01).Conclusion:STS pretreatment could pretect MI/RI,its effects maybe related with the improvement of membrane stability of myocardial myocytes.