摘要
为提高龙眼愈伤组织中单宁的提取率,采用超声波辅助浸提法,基于乙醇浓度(A)、超声波功率(B)与提取时间(C) 3个单因素试验,通过响应面试验优化设计,建立了龙眼愈伤组织单宁提取工艺。研究结果表明,龙眼愈伤组织中单宁的最佳提取条件为乙醇浓度61%、超声波功率140 W,提取时间54 min,提取温度60℃,在此工艺条件下,提取量为7.53 mg·g^(-1)。
In order to improve tannin extraction from longan callus,the effects of ethanol concentration,ultrasonic power and extraction time on the extraction of tannin were studied by ultrasound-assisted extraction.The tannin extraction procedure specification was then established using response surface methodology.The results show that optimized extraction of tannin(7.53 mg·g^-1)could be obtained under the condition of ethanol concentration 61%,ultrasonic power 140 W,extraction time 54 min and extraction temperature 60℃.
作者
刘生财
段俊朋
姚德恒
林玉玲
赖钟雄
LIU Shengcai;DUAN Junpeng;YAO Deheng;LIN Yuling;LAI Zhongxiong(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《浙江大学学报(理学版)》
CAS
CSCD
北大核心
2019年第1期58-64,共7页
Journal of Zhejiang University(Science Edition)
基金
国家自然科学基金资助项目(31572088)
福建省重大科技专项(2015NZ0002-1)
关键词
龙眼愈伤组织
单宁
超声波法
工艺优化
响应面分析
callus in Dimocarpus longan Lour
Tannins
ultrasound-assisted extraction
process optimization
response surface methodology