大鼠单核细胞趋化蛋白-1基因的生物信息学分析

Bioinformatics Analysis of Monocyte Chemotacticprotein-1 Gene in Rat

[目的]对大鼠单核细胞趋化蛋白-1(MCP-1)基因编码的蛋白质进行结构和性质分析。[方法]利用生物信息学方法对大鼠MCP-1基因编码的蛋白质的理化性质和结构进行分析,并分析该基因与其他物种的生物进化关系。[结果]大鼠MCP-1基因与小鼠的亲缘关系最近;大鼠MCP-1蛋白是一个稳定的亲水性碱性蛋白,分子式为C_(721)H_(1175)N_(197)O_(223)S_9,理论等电点为9.20,由8.78%的α-螺旋、35.81%的折叠延伸链、55.41%的无规则卷曲组成,有1个SCY结构域;该蛋白可能的信号肽剪切位点在第17位点处。[结论]MCP-1蛋白是一个稳定的亲水性分泌蛋白。该研究结果可为MCP-1的进一步研究提供理论依据。

[Objective]To investigate the structure and properties of Monocyte chemotacticprotein-1(MCP-1)gene encoding protein in rat.[Method]MCP-1 gene encoding protein of rat was analyzed including physicochemical property,structure,its evolution relationship with other species was inferred by bioinformatics analysis.[Result]MCP-1 protein in rat was a stable hydrophilic structural alkaline protein,the molecular formula was C 721 H 1175 N 197 O 223 S 9,which contained 8.78%a-helics,35.81%folding extending chain,55.41%random coil,it had a SCY domain.The possible signal peptide was located at the seventeenth amino acids residue.[Conclusion]MCP-1 protein in rat is a stable hydrophilic secretory protein.This study provides theoretical foundation for the further studies of MCP-1.