痰热清注射液联合亚胺培南和头孢哌酮/舒巴坦对广泛耐药肺炎克雷伯菌体外抑菌研究

In vitro antibacterial study of tanreqing injection combined with Imipenem and cefoperazone/sulbactam for extensively drug resistant Klebsiella pneumoniae

目的探讨痰热清注射液联合亚胺培南和头孢哌酮/舒巴坦对广泛耐药肺炎克雷伯菌的体外联合抑菌效果。方法应用肉汤稀释法测定痰热清注射液联合亚胺培南和头孢哌酮/舒巴坦对30株广泛耐药肺炎克雷伯菌的最低抑菌浓度(MIC),统计单独及联合用药的MIC分布,计算部分抑菌浓度(FIC)指数,判定联合用药的抑菌效果。FIC指数<0.5为协同作用,0.5～<1为相加作用,1～<2为无关作用,≥2为拮抗作用。结果痰热清注射液联合亚胺培南的FIC指数<0.5为43.3%,0.5～<1为30.0%,1～<2为20.0%,≥2为6.7%;痰热清注射液联联合头孢哌酮/舒巴坦的FIC指数<0.5为0,0.5～<1为13.3%,1～<2为33.3%,≥2为53.3%。结论痰热清注射液联合头孢哌酮/舒巴坦对广泛耐药肺炎克雷伯菌多表现为无关作用和拮抗作用;痰热清注射液联合亚胺培南多表现为协同作用和相加作用,对广泛耐药肺炎克雷伯菌的体外联合抑菌作用较好。

Objective To discuss antibacterial effect of Tanreqing injection combined with Imipenem and Cefoperazone/sulbactam on extensively drug resistant Klebsiella pneumoniae in vitro.Methods This experiment use the broth dilution method to determine the minimal inhibitory concentration(MIC)of the different concentrations of Tanreqing injection and Imipenem,Tanreqing injection and cefoperazone/sulbactam in 30 strains of the clinical extensively drug resistant Klebsiella pneumoniae,the fractional inhibitory concentration(FIC)index was calculated to determine the combined effect of the antibiotics.Results FIC index results of Tanreqing combined with Imipenem showed:FIC<0.5 is 43.3%,0.5<FIC≤1 is 30%,1<FIC≤2 is 20%,FIC>2 is 6.7%,FIC index results of Tanreqing combined with Cefoperazone/sulbactam showed:FIC<0.5 is 0%,0.5<FIC≤1 is 13.3%,1<FIC≤2 is 33.3%,FIC>2 is 53.3%.Conclusion The combined effect of tanreqing injection and cefoperazone/sulbactam is most irrelevant and antagonistic effect,the combined effect is worse.the combined effect of Tanreqing injection and Imipenem is most synergistic and additive effect,the combined effect is better.