苓泽合剂对急性痛风性关节炎大鼠血清IL-6、TNF-α含量的影响

Effect of Lingze Mixture on Serum Levels of IL-6 and TNF-α in Rats with Acute Gouty Arthritis

目的观察苓泽合剂对尿酸钠诱导的大鼠痛风性关节炎的抗炎作用,并探讨其作用机制。方法将80只Wistar大鼠随机分为空白组、模型组、芬必得组、苯溴马隆组、痛风定组及苓泽合剂低、中、高剂量组,每组10只。用尿酸钠诱导大鼠痛风性关节炎,测定血清中白细胞介素-6(interlenkin 6, IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)的含量。结果与模型组比较,各给药组血清中IL-6与TNF-α的含量均显著降低(P<0.01)。苓泽合剂中剂量组、高剂量组与低剂量组比较,血清中IL-6、TNF-α的含量明显下降(P<0.05)。结论苓泽合剂可抑制急性痛风性关节炎大鼠IL-6、TNF-α的表达,治疗痛风性关节炎。

Objective To explore the anti-inflammatory effect of Lingze Mixture on monosodium urate(MSU)crystal-induced gouty arthritis in rats and its mechanism of action.Methods Eighty Wistar rats were randomly divided into blank group,model group,fenbid group,benzbromarone group,Tongfengding group,and traditional Chinese medicine(TCM)Lingze Mixture groups(low,medium,and high doses),with ten rats in each group.A rat model of gouty arthritis was established by MSU crystals.Then the serum levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were determined.Results Compared with the model group,levels of IL-6 and TNF-αin serum in each model group were significantly decreased(P<0.01).Compared with the low-dose Lingze Mixture group,the medium-and high-dose Lingze Mixture groups had significantly reduced serum levels of IL-6 and TNF-α(P<0.05).Conclusion Lingze Mixture can inhibit the expression of IL-6 and TNF-αin rats with acute gouty arthritis,thus achieving the aim of preventing and alleviating gouty arthritis.