白芍不同提取方法及指纹图谱的研究

Study on Different Extraction Methods and Fingerprint of Paeonia lactiflora

目的:提高白芍有效成分提取率,优化提取工艺,完善质量控制方法,促进白芍加工过程标准化。方法:采用HPLC考察不同提取工艺对白芍药材中芍药苷、芍药内酯苷及没食子酸成分含量的影响;并对23批白芍药材进行指纹图谱分析。结果:超声时间为30 min时,50%甲醇对没食子酸、芍药内酯苷的提取率较高; 70%乙醇对芍药苷的提取率较高;指纹图谱共有模式中23批白芍样品生成19个共有峰,亳白芍和杭白芍指纹图谱之间差异较大。结论:不同提取溶剂对白芍药材中不同成分的溶出度不同,超声提取优于回流提取,提取时间为30 min时3种成分的提取量最高;同时实验所建立的指纹图谱重复性好,有助于全面、科学地评价白芍药材质量。

Objective: To improve the extraction rate of effective ingredients,optimize the extraction process,improve the quality control methods,and promote the standardization of Paeonia lactiflora processing. Methods: HPLC method was applied to investigate the effects of different extraction methods on the contents of paeoniflorin,albiflorin and gallic acid in P. lactiflora. Meanwhile the fingerprint analysis was used to analyze 23 batches of P. lactiflora. Results: Firstly,the extraction rate of gallic acid and albiflorin was higher in the condition of 50% methanol together with 30 minutes ultrasound time. Secondly,the extraction rate of paeoniflorin was higher in the condition of 70% ethanol together with 30 minutes ultrasonic time. Thirdly,there were 19 common peaks in the 23 batches of P. lactiflora showing a great difference between fingerprints of P. lactiflora in Bozhou and P. lactiflora in Hangzhou. Conclusion: The extracting solvents has a certain impact on the dissolution rate of different components in P. lactiflora. Ultrasonic extraction is superior to reflux extraction. The extraction rate of the three components is the highest with thirty minutes. The fingerprint established by the experiment is well reproducible,which can contribute to a comprehensive and scientific evaluation of the quality of P. lactiflora.