西酞普兰对小鼠角膜上皮细胞和泪膜功能的影响

Influence of Citalopram on tear film function and corneal epithelium in mice

目的研究西酞普兰对小鼠泪膜功能和角膜上皮细胞的影响。方法选取36只BABL/c雄性小鼠随机分为A、B、C 3组,A组:空白对照组(不进行任何处理);B组:生理盐水组,以10 mg/kg腹腔注射生理盐水;C组:西酞普兰组,以10 mg/kg腹腔注射西酞普兰。分别在实验第1、4和7天时行泪液分泌试验(SIT)、泪膜破裂时间(BUT)检测,同时于实验第7天进行角膜荧光素染色(FL),取眼球做扫描电镜及HE染色观察角膜上皮损伤情况。结果 (1) 3组不同时间点的SIT、BUT、FL有差异(F=9.749、10.427和6.752,P=0.008、0.019和0.032);(2) 3组的SIT、BUT、FL有差异(F=11.234、14.842和5.337,P=0.002、0.012和0.029);(3) 3组的SIT、BUT、FL变化趋势有差异(F=16.438、11.753和16.431,P=0.000、0.001和0.002)。干预后第7天,3组上皮空泡细胞数比较,差异有统计学意义(F=7.749,P=0.013)。3组微绒毛数量比较差异无统计学意义(F=0.917,P=0.092)。结论西酞普兰会损伤小鼠泪膜功能的稳定性,但对角膜上皮细胞无影响。

Objective To investigate the effect of Citalopram on tear film function and corneal epithelium in mice.Methods A total of 36 BABL/c male mice were randomly divided into group A,group B and group C.Mice in group A received no treatments.Mice in group B received saline (10 ml/kg) via intraperitoneal injection.Mice in group C were intraperitoneally injected with Citalopram (10 mg/kg) on daily basis.Schirmer I test (SIT) and the break-up time (BUT) were carried out on day 1,4 and 7 post insults.On the 7th day,corneal fluorescein staining (FL) was carried out,and histological analysis of eyeballs of mice were evaluated by scanning electron microscope (SEM) and HE staining.Results SIT,BUT and FL were significantly different on day 1,4,and 7 in three groups (F=9.749,10.427 and 6.752,P=0.008,0.019 and 0.032,respectively).SIT,BUT and FL were statistically different among group A,group B and group C(F=11.234,14.842 and 5.337,P=0.002,0.012 and 0.029,respectively).Seven days after the intervention,numbers of epithelial cavitation cells in group A,group B and group C were obviously different (F=7.749,P=0.013).Mice in group C experienced villi decrease without significant difference among groups (F=0.917,P=0.092).Conclusions Citalopram damages the function of tear film while exerts no effect on corneal epithelial tissue in mice model.