体外培养乳牙牙髓干细胞向血管内皮细胞定向分化的实验研究

Study of differentiation of stem cells from deciduous teeth into vascular endothelial cell

目的研究体外培养乳牙牙髓干细胞向血管内皮细胞的定向分化。方法选取南方医科大学口腔医院29例健康儿童的滞留乳牙,在4 h内进行乳牙牙髓干细胞的原代提取。采用免疫磁珠分选法进行细胞分选,并对分选出的细胞进行特定的血管内皮细胞定向诱导。对乳牙牙髓干细胞及血管内皮细胞定向诱导后的细胞进行镜下形态观察、血管生成实验,以及免疫表型鉴定。结果镜下形态观察显示,经过血管内皮定向诱导的乳牙牙髓干细胞呈典型血管内皮细胞的形态特征;血管生成实验表明,经过血管内皮定向诱导的乳牙牙髓干细胞可呈现明显的血管样结构;免疫表型鉴定显示经过血管内皮定向诱导的乳牙牙髓干细胞血管内皮细胞表面抗原呈阳性。结论乳牙牙髓干细胞可定向分化为血管内皮细胞。

Objective To induce differentiation of stem cells from human exfoliated deciduous teeth into vascular endothelial cell.Methods Deciduous teeth from healthy children were collected in our hospital.Human exfoliated deciduous teeth were primarily isolated within 4 hours.The stem cells from human exfoliated deciduous teeth were purified by magnetic cell sorting and were induced to vascular endothelial cells.Determination of the differentiated vascular cells were identified by morphology,angiogenic study and immunophenotype.Results Vascular endothelial cells showed typical cell morphological character.The angiogenic research showed that vascular endothelial cells could present obvious vessel-like structure.The identification of immunophenotype showed positive surface molecules of vascular endothelial cells.Conclusions The stem cells from human exfoliated deciduous teeth can be differentiated into vascular endothelial cells.