子宫内膜癌组织microRNA-944的表达及临床意义

Role of microRNA-944 in patients with endometrial carcinoma

目的探讨子宫内膜癌组织micro RNA-944(mi R-944)的表达及其与子宫内膜癌(EC)患者临床病理特征及预后的关系,并分析mi R-944对患者的早期诊断价值。方法采用实时荧光定量聚合酶链反应(q RT-PCR)检测EC癌组织(80例)和正常子宫内膜组织(30例)中mi R-944的表达情况,分析mi R-944表达与EC患者临床病理特征之间的关系。采用Kaplan-Meier生存曲线法分析mi R-944表达与EC患者预后的关系,并采用受试者操作特征曲线(ROC)分析mi R-944对EC的早期诊断价值。结果 q RT-PCR结果显示,mi R-944在EC癌组织中的表达水平高于正常子宫内膜组织(P <0.05)。在EC癌组织中,mi R-944表达水平与肿瘤肌层浸润深度、有无淋巴转移、有无远处转移及国际妇产联盟(FIGO)分期相关(P <0.05),而与患者年龄、绝经状态、病理分型和组织分级无关(P>0.05)。Log-rank检验结果显示,mi R-944高表达组患者的生存时间较mi R-944低表达组降低(P <0.05)。ROC分析结果显示,mi R-944的曲线下面积(AUC)为0.784。结论EC癌组织中mi R-944呈高表达,且其高表达与EC患者不良预后密切相关,mi R-944可作为EC早期诊断和预后预测的潜在靶点。

Objective To investigate expression of microRNA-944 (miR-944) in patients with endometrial carcinoma (EC),and to determine its clinical significance.Methods The expression level of miR-944 in 80 cases of EC cancer tissues and 30 cases of normal endometrial tissues were measured by real-time quantitative fluorescence PCR (real-time PCR) test.Relationship between the expression of miR-944 and the clinical characteristics of patients with EC was analyzed.Results RT-PCR showed that the expression level of miR-944 in EC cancer tissue was significantly higher than that in normal endometrium (P<0.05).Expression of miR-944 was correlated to the depth of tumor myometrium infiltration,lymph node metastasis,distant metastasis and FIGO stage (P<0.05).Log-rank test showed that the survival time of the patients with high expression of miR-944 was decreased obviously compared with that in patients with low expression group of miR-944 (P<0.05).ROC analysis showed that the area under the curve of miR-944 (AUC) was 0.784.No obvious correlation was identified between miR-944 and age,menopausal status,pathological classification,or histological grade (P>0.05).Conclusions High expression of miR-944 in EC cancer tissue relates to the poor prognosis,which may be used as a potential diagnostic and prognostic biomarker.