短柱八角中二萜类成分及其抗炎活性

Diterpenes from Illicium brevistylum and their anti-inflammatory activities

目的研究短柱八角Illicium brevistylum A. C. Smith中二萜类成分及其抗炎活性。方法短柱八角80%乙醇提取物石油醚部位采用硅胶柱、反相ODS、Sephadex LH-20进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。用LPS诱导的293-Nf-κB-luc细胞模型对其进行抗炎活性检测。结果从中分离得到9个二萜类化合物,分别鉴定为异海松酸(1)、脱氢枞酸(2)、3,16α-二羟基-贝壳杉烷(3)、8,11,13,15-abietatetraen-19-oic acid (4)、methyl-16-nor-15-oxodehydroabietate (5)、12-羟基-脱氢枞酸(6)、3,4-开环海松烷-4,8 (9),15 (18)-三烯-3-酸甲酯(7)、15-羟基取代松香烷甲酯(8)、9β,13β-endoperoxide-abieta-8 (14)-en-18-oic acid (9)。化合物4～6、8均能降低LPS诱导的293-Nf-κB-luc细胞荧光强度。结论所有化合物均首次从该植物中分离得到,化合物3、7首次从该属植物中分离得到,化合物4～6、8均表现出较强的抗炎活性。

AIM To study the diterpenes from Illicium brevistylum A. C. Smith and their anti-inflammatory activities. METHODS The petroleum ether fraction of 80% ethanol fraction extract from I. brevistylum was isolated and purified by silica,reversed phase ODS and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data. Their anti-inflammatory activities were evaluated by LPS-induced 293-Nf-κB-luc cell model. RESULTS Nine diterpenes were isolated and identified as isopimaric acid( 1),dehydroabietic acid( 2),3,16α-dihydroxy-kaurane( 3),8,11,13,15-abietatetraen-19-oic acid( 4),methyl-16-nor-15-oxodehydroabietate( 5),12-hydroxydehydroabietic acid( 6),3,4-secopimara-4,8( 9), 15( 18)-triene-3-acid-methylester( 7), methyl-15-hydroxydehydroabitetate( 8), 9β, 13β-endoperoxide-abieta-8( 14)-en-18-oic acid( 9). Compounds 4-6,8 significantly suppressed the fluorescence intensity in LPS induced 293-Nf-κB-luc cell model. CONCLUSION All the compounds are isolated from this plant for the first time,compounds 3 and 7 are first isolated from genus Illicium,and compounds 4-6,8 show good antiinflammatory activities.