摘要
利用淀粉选择性培养基从土壤中分离得到1株有明显水解圈的淀粉酶产生菌Y1,经形态学、16SrDNA鉴定其为蜡样芽孢杆菌(Bacillus cereus);采用碘比色改良法测定Y1淀粉酶酶活,其酶活为140U/m L。
A α-amylase-producing strain was isolated from soil with starch-selective medium,named Y1. Y1 was identified through morphology observation and 16SrDNA. As a result,strain Y1 was identified as Bacillus cereus. The α-amylase activity of fermentation broth was 140U/m L determined by modified iodine colorimetry.
作者
陈燕
刘盾
陈玄阳
黄家驷
王珊珊
程爽
Chen Yan;Liu Dun;Chen Xuanyang;Huang Jiasi;Wang Shanshan;Cheng Shuang(School of Biological and Chemical Engineering,Nanyang Institute of Technology,Nanyang 473004,China;Henan Key Laboratory of Industrial Microbiology Resources and Fermentation Technology,Nanyang 473004,China)
出处
《山东化工》
CAS
2018年第24期75-77,共3页
Shandong Chemical Industry
基金
南阳理工学院本科专业核心课程改革专项研究项目(HXKC2016114)
