大蒜素联合番茄红素对高血压大鼠靶器官损伤及抗氧化能力的影响

Effects of allicinin combined with lycopene on target organ damage and the antioxidative status in hypertensive rats

目的探讨大蒜素(allicin,All)联合番茄红素(lycopene,Lyc)对自发性高血压引起的靶器官损伤及氧化应激的保护作用。方法 10周龄雄性自发性高血压大鼠(SHR)随机分为4组(n=8):SHR组、SHR+All组、SHR+Lyc组和SHR+All+Lyc组。各组每天采用腹腔注射15mg/(kg·d)All或/和7.5mg/(kg·d)Lyc灌胃干预6周,以腹腔注射等体积生理盐水的WKY大鼠作为对照。6周后,HE染色检测胸主动脉和肾脏的病理学改变,并对肾组织病理变化进行Paller评分;ELISA法检测血清和胸主动脉中的氧化应激水平及抗氧化指标;DHE荧光染色检测胸主动脉中的超氧阴离子(O-2)含量。结果 SHR+All+Lyc组与SHR组、SHR+All组及SHR+Lyc组相比,显著改善SHR大鼠的主动脉壁增厚及肾脏炎性损伤(P<0.05),并可使SHR大鼠血清和主动脉中的抗氧化指标超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH)及动脉中的过氧化氢酶(CAT)活性明显升高(P<0.05);与SHR大鼠相比,All联合Lyc可显著减少高血压诱导的丙二醛(MDA)和O-2水平升高(P<0.05)。结论大蒜素联合番茄红素可能通过增强抗炎及抗氧化的调节机制来降低高血压诱导的靶器官损伤和氧化应激。

Objective To investigate the effects of allicin(All)in combination with lycopene(Lyc)against target organ damage and oxidative stress induced by hypertension in spontaneously hypertensive rats(SHRs).Methods SHRs aged 10 weeks were randomly divided into four groups(n=8 in each group):SHR,SHR+All,SHR+Lyc,and SHR+All+Lyc.SHR in SHR+All,SHR+Lyc and SHR+All+Lyc groups were intraperitoneally or intragastrically administered with 15 or 7.5 mg/(kg·d)of All and Lyc at the same time daily for 6 weeks.Male WKY rats were intraperitoneally injected with the same volume of normal saline and served as normotensive controls(WKY group).Six weeks later,histopathological changes of thoracic aorta and kindeys were evaluated by HE staining and according to Paller s method.The indexes of oxidation(MDA)and antioxidants(SOD,CAT and GSH)in serum or thoracic aorta were determined by enzyme-linked immunosorbent assay(ELISA).Production of intracellular superoxide anion was stained by dihydroethidium(DHE)staining and detected using laser scanning confocal microscope.Results SHR+Lyc+All group significantly ameliorated arterial wall thickening and inflammatory injury of the kindey in SHR compared with SHR+All group and SHR+Lyc group.Administration of All combined with Lyc significantly increased activities of antioxidative enzymes(SOD and GSH)and the content of CAT in aortas when compared with those of SHR and SHR receiving All or Lyc administration alone(P<0.05).In addition,the combined treatment of All with Lyc also significantly reduced the contents of MDA and O2^-of SHR compared to SHR(P<0.05).Conclusion This combination of All and Lyc can reduce target organ damage and level of oxidative stress induced by hypertension,which may be attributed to their regulations on ROS level,antioxidant and anti-inflammatory abilities.