高脂饮食诱导建立小鼠高脂血症模型

The establishment of hyperlipidemic models induced by high fat diets in mice

目的探讨不同配方的高脂饮食对2种不同种系小鼠的高脂血症成模的影响。方法用4种不同配方的高脂饲料对昆明种小鼠和C57BL/6小鼠长期喂饲后,考察体质量和内脏脂肪含量,检测血和肝脏中三酰甘油和总胆固醇的水平。结果不同配方的高脂饲料均可使2种小鼠血浆中总胆固醇水平升高(高脂饲料1对昆明种小鼠:1 892±83mg·L-1vs 691±32mg·L-1,P<0.01),但却降低了小鼠血浆中三酰甘油水平(高脂饲料1对昆明种小鼠:1 125±116mg·L-1vs 2 061±138mg·L-1,P<0.01),高脂饲料中胆固醇和胆酸钠的有无与小鼠肝脏中总胆固醇和三酰甘油水平呈明显正相关,与血浆中三酰甘油水平呈负相关,对血浆胆固醇的水平影响不大。结论采用高脂饮食长期喂饲能成功建立小鼠高胆固醇血症模型,但无法建立小鼠高三酰甘油血症模型。

Objective To study the effects of different high fat diets(HFD)on hyperlipidemic models in 2 strains mice.Methods To feed C57BL/6 and Kunming mice with 4 different HFD for several weeks,then body weights,visceral fats,total cholesterol(TC)and triglyce ride(TG)in blood and in livers were measured.Results 4 different HFD could increase blood TC,liver TC and liver TG in 2 stains mice(blood TC in Kunming mice induced by type 1 HFD and by normal diet:1 892±83 mg·L^-1 vs 691±32 mg·L^-1,P<0.01)and inhibited blood TG levels in C57BL/6 and Kunming mice(blood TG in Kunming mice induced by type 1 HFD and by normal diet:1 125±116 mg·L^-1 vs 2 061±138 mg·L^-1,P<0.01).Cholesterol and sodium cholate in HFD positively regulated liver TC and liver TG and negatively regulated blood TG level in Kunming mice.Conclusion Hypercholesterolemia could be successfully established in mice fed with HFD,however,hypertriglyceridemia could not be induced by HFD in C57BL/6 and Kunming mice.