CO_2气腹对妊娠中晚期大鼠血浆前列腺素E2水平的影响

Effect of carbon dioxide pneumoperitoneum on plasma prostaglandin E2 level in rats with medium and late pregnancy

目的观察CO_2气腹对妊娠中晚期大鼠血浆前列腺素E2(PGE2)水平的影响。方法 48只妊娠SD大鼠随机分为孕中期对照组、1 h气腹组、2 h气腹组及孕晚期对照组、1 h气腹组和2 h气腹组,用酶联免疫吸附法检测血浆PGE2水平。结果孕中期、晚期1 h气腹组血浆PGE2水平与同期对照组差异无统计学意义(P>0.05),而孕中期、晚期2 h气腹组血浆PGE2水平均显著高于同期对照组(P<0.05)。结论 CO_2气腹2 h可提高孕中、晚期大鼠血浆PGE2水平。

Objective To study the effect of carbon dioxide(CO2)pneumoperitoneum on plasma prostaglandin E2(PGE2)content in rats with medium and late pregnancy.Methods Forty-eight SD pregnant rats were randomized to medium and late pregnancy groups,and then subdivided into control and 1-h,2-h pneumoperitoneum subgroups.Plasma PGE2 level was measured by enzyme-linked immunosorbent assay.Results Compared with control group,plasma PGE2 content was increased in 2-h but not in 1-h pneumoperitoneum subgroups(P<0.05)despite of medium and late pregnancy.Conclusion The 2-h CO2 pneumoperitoneum can elevate plasma PGE2 level in rats with medium and late pregnancy.