补肾活血方对动脉粥样硬化新西兰兔miRNA-217/Sirt1/FoxO1信号通路的影响

The Influence of Bushen Huoxue Recipe on miRNA-217/Sirt1/FoxO1 Signaling Pathway in New Zealand Rabbits with Atherosclerosis

目的观察补肾活血方对动脉粥样硬化新西兰兔MicroRNA-217(miRNA-217)/沉默信号调节器1(Sirt1)/叉头转录因子(FoxO1)信号通路的影响。方法将新西兰兔44只随机分为对照组、模型组、补肾活血方组和阿托伐他汀钙组,除对照组外,其余3组高脂饲料喂养16周,灌胃8周后实时荧光定量PCR检测miRNA-217基因表达水平,免疫印迹实验检测Sirt1、FoxO1蛋白表达水平。结果与模型组相比,补肾活血方组和阿托伐他汀钙组miRNA-217表达降低(P <0.01),Sirt1、FoxO1表达增高(P <0.01)。补肾活血方组miRNA-217水平低于阿托伐他汀钙组(P <0.05),Sirt1蛋白水平高于阿托伐他汀钙组(P <0.05)。结论补肾活血方可能通过调控miRNA-217/Sirt1/FoxO1信号通路延缓内皮细胞衰老从而起到抗动脉粥样硬化作用。

Objective To observe the effect of Bushen Huoxue recipe(BHR)on the miRNA-217/silencing signal regulator 1(Sirt1)/fork-head transcription factor(FoxO1)signaling pathway in atherosclerosis of New Zealand rabbits.Methods Forty-four New Zealand rabbitswere randomly divided into control group,model group,BHR group,and atorvastatin group.Except the control group,the rabbits were fedwith high-fat diet for 16 weeks.The expression of miRNA-217 gene was detected by Realtime-PCR after 8 weeks of intragastric adminis-tration.The expression of Sirt1 and FoxO1 protein was detected by western blot.Results Compared with the model group,the expressionof miRNA-217 in BHR group and atorvastatin group decreased(P <0.05),while the expression of Sirt1 and FoxO1 increased(P <0.05).Among them,the levels of miRNA-217 in BHR group were lower than those in atorvastatin group(P <0.05),and the levels of Sirt1 pro-tein were higher than those in atorvastatin group(P <0.05).Conclusion BHR may play an anti-atherosclerotic role by delaying endothe-lial cell senescence through regulating the miRNA-217/Sirt1/FoxO1 signaling pathway.