保肝汤对小鼠四氯化碳致慢性化学性肝损伤的保护作用

Protective effect of Baogan decoction on chronic chemical liver injury induced by carbon tetra chloride in mice

[目的]研究保肝汤对四氯化碳(CCl4)致慢性化学性肝损伤的保护作用。[方法]将84只昆明小鼠随机分为正常组、模型组、护肝片组、保肝汤高、中、低剂量组共6组,每组14只。除正常组外,其余各组分别在实验第1、3、10、16、22、25、28天腹腔注射0.5%CCl4;于造模后第10天开始灌胃,其中正常组和模型组给予蒸馏水灌胃,护肝片及保肝汤高、中、低剂量组给予灌胃药液。第30天灌胃2 h后称质量、处死,并收集各组肝组织及血清标本,检测指标含量,计算肝脏指数并进行肝组织病理学检查。[结果]与正常组比较,模型组丙氨酶转氨酶(ALT)、天门冬氨酶氨基转移酶(AST)、丙二醛(MDA)明显升高(P<0.05)。模型组肝组织病理学,可见多处大片肝细胞脂肪变性,并见多处灶状坏死;与模型组比较,保肝汤高、中、低剂量组ALT、AST降低,AST/ALT升高(P<0.05),保肝汤中、低剂量组MDA降低(P<0.05);保肝汤各剂量组明显改善肝组织及形态结构。[结论]保肝汤能够减轻CCl4对肝细胞的损伤程度,以保肝汤中剂量组作用最优;作用机制与抑制肝损伤后脂质过氧化反应有关。

[Objective] To study the protective effect of Baogan decoction on chronic chemicai liver injury of mice induced by carbon tetrachloride(CCl4).[Methods] A total of 84 Kunming mice were randomly divided into normal group,model group,hugan tablet group,baogan decoction high,middle and low dose group,with fourteen in each group.Except for the normal group,the other groups were injected with 0.5%CCl4 at the abdominal cavity on the first day,the third day,the 10 th day,the 16 th day,the 22 th day,the 25 th day and the 28 th day in the experimental period.The 10 th day of molding began to Intragastric administration.Normal group and model group were given intragastric administration of distilled water.Hugan tablet group,high dose of baogan decoction,middle dose of baogan decoctionand low dose of baogan decoction intragastric administration of drugs.After intragastric administration of 30 d,mice were weighed,killed,and liver tissues and serum samples were collected.Then test the content of the experimental indicators,and calculate the liver index and hepatic tissue pathological examination.[Results] Compared with the normal group,the ALT,AST and MDA in the model group increased significantly(P<0.05).Pathological changes in liver tissue of model group showed the arrangement of liver cells was disordered.Many large hepatic cells showed fatty degeneration and multiple foci of necrosis.Compared with the model group,the ALT,AST in the high,middle and low dose groups decreased(P<0.05),and the AST/ALT increased(P<0.05),and the MDA in the middle and low dose groups decreased(P <0.05).Liver tissue and morphological structure were significantly improved in each dose group.[Conclusion] Baogan decoction can alleviate the damage degree of CCl4 on liver cells,in which the hepatoprotective effect of the middle dose group is the best.The mechanism of action is related to the inhibition of lipid peroxidation after liver injury.