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不同缺血后处理时间模式对小鼠肺缺血再灌注损伤保护作用的研究 被引量:1

Protective effection of different model of ischemic postconditioning on lung ischemia-reperfusion injury in rats
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摘要 (1)目的探讨不同缺血后处理(IPost-C)时间模式对小鼠肺缺血再灌注损伤的保护作用。(2)方法将30只大鼠随机分为5组:对照组(S组,n=6)只暴露肺门;IPost-C组根据缺血30分钟后处理时间周期不同分为Ⅰ、Ⅱ、Ⅲ、Ⅳ组:15s灌注/15s再缺血(IPost-CⅠ组)、30s灌注/30s再缺血(IPost-C Ⅱ组)、45s灌注/45s再缺血(IPost-C Ⅲ组)、60s灌注/60s再缺血(IPost-C Ⅳ组),即开胸分离出左肺门,放置阻断带,阻断30分钟后,分别给予3个时间周期的后处理,然后完全恢复血液再灌注2小时;观察不同方式下肺组织湿/干(W/D)比,WB方法测定IL-6、IL-10的含量,TUNEL法测定细胞凋亡百分比。(3)结果与对照组比较,肺组织W/D、IL-6、IL-10的含量和肺组织病理改变及细胞凋亡百分比在(IPost-C)各组均有明显变化,差异有统计学意义(P <0.05);但IPost-C Ⅱ和IPost-C Ⅲ两组之间差异无统计学意义(P>0.05),且肺组织病理改变亦较轻。(4)结论在肺缺血再灌注损伤后处理模式中,30s灌注/30s再缺血和45s灌注/45s再缺血组均有较好的肺组织保护作用,但前者肺组织病理变化更轻,故临床上选取30s灌注/30s再缺血的缺血后处理模式最为合适。 Objective To explore the time model of the ischemic postconditioning for the lung ischemia-reperfusion injury in rats.Methods A total of 30 rats were randomly divided into 5 groups:normal group(S,n =6)only exposed hilus of the left lung.Ischemia post-conditionging group(IPost-C)Ⅰ(n=6)three successive cycles for 15 sreperfusion and 15 socclusion before restoring full perfusion were underwent.IPost-C Ⅱ(n =6)three successive cycles for 30 sreperfusion and 30 socclusion before restoring full perfusion were done.IPost-C Ⅲ(n =6)three successive cycles for 45 s reperfusion and 45 socclusion before restoring full perfusion were acted.IPost-C Ⅳ(n =6)three successive cycles for 60 sreperfusion and 60 socclusion before restoring full perfusion were performed.The histopathological changes,wet/dry(W/D)weight ratio,apoptosis index were observed.The concentration of interleukin-6(IL-6),interleukin-10(IL-10)in the lung tissue was measured by an enzyme-linked immunosorbent assay.Results Compared with S group,the changes of histopathology,W/D,IL-6,IL-10 in the lung tissue on 4IPost-C groups were significantly difference(P <0.05).There were no statistical difference between IPost-C Ⅱ and IPost-C Ⅲ group(P >0.05).ConclusionThe model of the ischemic postconditioning 30 sand 45sreperfusion/occlusion maybe bring a reducing and protection of the lung I/R injury.The appropriate choice of 30sreperfusion/occlusion is advisable and possible for protection of the lung I/R injury on the clinical practice.
作者 兰峻斌 高木火 白玉坤 高毅哲 吴艺根 段明科 LAN Junbin;GAO Muhuo;BAI Yukun(Department of Thoracic Surgery,Xiamen Haicang Hospital,Xiamen 361026,China)
出处 《华北理工大学学报(医学版)》 2019年第1期1-6,共6页 Journal of North China University of Science and Technology:Health Sciences Edition
基金 福建省医学创新课题(编号:2009-CXB-62)
关键词 缺血再灌注损伤 缺血后处理模式 Lung Ischemia-reperfusion injury Model of the ischemic postconditioning
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