胶质母细胞瘤中内源竞争RNA网络介导的长链非编码RNAs功能研究

Study on the function of competing endogenous RNA network-mediated long non-coding RNAs in glioblastoma

胶质母细胞瘤是人类常见的且恶性程度极高的一种脑部肿瘤。大量研究表明长链非编码RNAs(long non-coding RNAs,lncRNAs)在GBM的进程中发挥着重要的作用。本研究通过整合lncRNA-miRNA/mRNA-miRNA关系对和GBM表达谱数据,构建GBM相关的内源竞争RNA (competing endogenous RNA,ceRNA)背景并分析网络中lncRNAs和mRNAs的拓扑属性。然后,我们筛选度和中介中心性前5%的节点作为hub节点,进一步构建GBM中lncRNAs介导的hub-ceRNA网络并分析预测网络中lncRNAs的功能,从而识别GBM诊断和治疗相关的靶点基因。本研究进一步加深对GBM中lncRNAs功能研究的认识,识别出新的GBM风险基因和潜在的治疗靶点。

Glioblastoma is a common and highly malignant brain tumor.Numerous studies have shown that lncRNAs play an important role in the process of GBM.In this study,we integrated the lncRNA-miRNA/mRNA-miRNA pairs and GBM expression profile to construct a GBM-related ceRNA background network and analyzed the topological properties of lncRNAs and mRNAs in the network.Next,we screened the top 5%of degree and betweenness centrality as the hub nodes to further construct the lncRNAs-mediated hub-ceRNA network in GBM and analyzed the function of lncRNAs in the network to identify the target genes involved in GBM diagnosis and treatment.This study further deepens the understanding of the functional studies of lncRNAs in GBM and identifies new GBM risk genes and potential therapeutic targets.