乳腺癌组织微小RNA-218-5p的表达及其临床意义

Expression and clinical significance of microRNA-218-5p in breast cancer tissues

目的探讨微小RNA-218-5p(miR-218-5p)在乳腺癌组织中的表达及临床意义。方法收集本院2013年1月至2015年12月女性乳腺癌患者手术切除的92对癌组织和癌旁组织,采用实时定量PCR(QPCR)检测以上组织中miR-218-5p的表达并比较miR-218-5p在癌组织和癌旁组织中的表达差异,进一步分析miR-218-5p表达与乳腺癌临床病理特征(年龄、肿瘤大小、淋巴结转移、TNM分期、绝经、组织学分级、ER、PR、HER-2和Ki-67表达)的关系,采用门诊或电话随访并比较miR-218-5p低表达组(<均值)和高表达组(≥均值)的预后情况。结果 QPCR检测结果显示,乳腺癌组织的miR-218-5p为0. 315±0. 150,低于癌旁组织的1. 010±0. 298,差异有统计学意义(P<0. 05)。乳腺癌患者miR-218-5p水平与年龄、淋巴结转移、绝经、ER和PR表达均无关,而与肿瘤大小、TNM分期、组织学分级、HER-2和Ki-67表达有关(P<0. 05)。全组随访14～60个月,中位随访45. 0个月,中位总生存期(OS)为57. 0个月,其中miR-218-5p高表达组的中位OS>60. 0个月,高于低表达组的51. 0个月,差异有统计学意义(P<0. 05)。单因素分析发现,乳腺癌患者预后还与年龄、肿瘤大小、淋巴结转移、TNM分期、组织学分级和HER-2表达有关,差异有统计学意义(P<0. 05)。结论 MiR-218-5p在乳腺癌中低表达且与肿瘤的发生发展和预后有一定关系,miR-218-5p低表达者的预后较差,该指标在乳腺癌筛查及预后预测上有一定潜在价值。

Objective To investigate the expression and clinical significance of microRNA-218-5p( miR-218-5p) in breast cancer tissues. Methods From January 2013 to December 2015,92 pairs of cancerous and paracancerous tissues surgically removed from patients with breast cancer were collected. Real-time quantitative PCR( QPCR) was used to detect the expression of miR-218-5p in the above tissues. The expression difference of miR-218-5p between cancer tissues and adjacent tissues was compared. The relationship between the miR-218-5p expression and clinicopathological characteristics of breast cancer( age,tumor size,lymph node metastasis,TNM stage,menopause,histological grade,ER,PR,HER-2 and Ki-67 expression) were compared. The outpatient or telephone follow-up was used to compare the prognosis of the high expression group( ≥mean value) and the low expression group( <mean value) of miR-218-5p level. Results The results of QPCR showed that the expression of miR-218-5p in breast cancer tissues was 0. 315±0. 150,lower than 1. 010±0. 298 in adjacent tissues,and the difference was statistically significant( P< 0. 05). The levels of miR-218-5p in patients with breast cancer were independent of lymph node metastasis,age,menopause,ER expression and PR expression,and significantly related with tumor size,TNM stage,histological grade,HER-2 expression and Ki-67 expression( P<0. 05). The patients were followed up for 14 to 60 months with a median follow-up of 45. 0 months and a median overall survival( OS) of 57. 0months. The median OS of the group with high expression of miR-218-5p was more than 60. 0 months,higher than 51. 0 months of the group with low expression of miR-218-5p( P<0. 05). Univariate analysis also found that the prognosis of breast cancer patients was significantly different from age,tumor size,lymph node metastasis,TNM stage,histological grade and HER-2 expression( P < 0. 05).Conclusion MiR-218-5p expression is low in breast cancer tissues and has a certain relationship with the occurrence,development and prognosis of breast cancer. The prognosis of patients with low expression of miR-218-5p is poor. This factor has certain potential value in breast cancer screening and prognosis prediction.