PEI-CDs复合物的制备及其在药物传输中的应用研究

Preparation of New Carbon Quantum Dot Complex and Its Application in Drug Transport

癌症是21世纪威胁人类健康的三大杀手之一。目前,治疗癌症的药物由于缺乏靶向性和选择性对正常细胞和组织也会造成严重损害。靶向性载药体系的构建是解决这一难题的关键。碳点(Cardon dots,CDs)是近年来发展起来的一种新型荧光纳米材料,因其具有无毒性、优良的生物兼容性、良好的发光性能等优点,在肿瘤标记及药物的靶向输送中具有重大的应用潜力。本实验以枸杞为碳源,采用水热法制备碳量子点,通过静电作用将聚乙烯亚胺(PEI)修饰在碳量子点表面,形成PEI-CDs复合物,在室温下通过物理吸附的手段使盐酸阿霉素(Dox)组装到PEI-CDs复合物表面,形成PEI-CDs-Dox型荧光载药系统,并对该载药体系进行体外释放模拟实验。实验结果表明,阿霉素的负载显著增强了碳点复合物的荧光性能,24h释放率可达90%,随着药物的释放,体系的荧光强度逐渐下降,据此可以将其进一步应用于细胞靶向成像及药物靶向监测。

Cancer is one of the three major killers of human health in the 21 st century.Currently,drugs for treating cancer also cause serious damage to normal cells and tissues due to lack of targeting and selectivity.The construction of targeted drug delivery systems is the key to solving this problem.Carbon dots are a new type of fluorescent nanomaterials developed in recent years.Because of their non-toxicity,excellent biocompatibility and good luminescence properties,carbon dots have great application potential in tumor markers and targeted delivery of drugs.In this paper,medlaris used to prepare carbon quantum dots by hydrothermal method,and polyethyleneimine(PEI)was modified on the surface of carbon quantum dots by electrostatic action to form PEI-CDs complex.Doxorubicin hydrochloride(Dox)was assembled onto the surface of PEI-CDs complex by physical adsorption at room temperature to form a PEI-CDs-Dox type fluorescent drug delivery system,and the drug delivery system was subjected to in vitro release simulation experiments.The experimental results show that the loading of doxorubicin significantly enhances the fluorescence performance of the carbon dots complex.The 24 hrelease rate can reach 90%.With the release of the drug,the fluorescence intensity of the system gradually decreases,which can be further applied to cell-targeted imaging and drug-targeted monitoring.