基于UPLC-Q-Orbitrap MS法分析补肺益肾方的化学成分

Identification of Chemical Constituents of Bufei Yishen Formula by UPLC-Q-Orbitrap MS

基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap MS)法,应用Mass Frontier质谱解析软件,对补肺益肾方的化学成分进行了定性分析。采用Phenomenex kinetex C_(18)(100 mm×2. 1 mm,2. 6μm)色谱柱,以1‰甲酸水-乙腈为流动相梯度洗脱,流速为0. 2 mL·min^(-1),柱温为25℃,进样量为5μL;应用Mass Frontier 6. 0软件对采集的碎片离子进行匹配,并结合文献报道,共鉴定了补肺益肾方的242个化学成分。在正离子模式下鉴定205个化学成分,其中人参10个,黄芪17个,赤芍28个,浙贝母9个,炒紫苏子63个,陈皮9个,矮地茶8个,酒萸肉7个,醋五味子26个,淫羊藿(炙)28个;负离子模式下鉴定37个成分,其中人参23个,黄芪14个。UPLC-Q-Orbitrap MS法可全面快速分析补肺益肾方的化学成分,为补肺益肾方的药效物质研究提供了基本数据。

A method was developed for the identification of chemical constituents in Bufei Yishen formula by UPLC-Q-Orbitrap MS with Mass Frontier analysis software.The sample was separated on a Phenomenex kinetex C 18 column(100 mm×2.1 mm,2.6μm)by gradient elution with 1‰formic acid-acetonitrile as mobile phase at a flow rate of 0.2 mL·min^-1,a column temperature of 25℃and an injection volume of 5μL.Total of 242 chemical components in Bufei Yishen formula were confirmed by using Mass Frontier 6.0 workstation to match the collected fragment ions,combined with the literature report,in which 205 chemical components were confirmed in positive ion mode,including 10 from Ginseng Radix Rhizoma,17 from Astragali Radix,28 from Paeoniae Rubra Radix,9 from Fritillariae Thunbergii Bulbus,63 from Perillae Fructus,9 from Citri Reticulatae Pericarpium,8 from Ardisiae Japonicae Herba,7 from Corni Fructus,26 from Schisandrae Chinensis Fructus and 28 from Epimedii Herba.While 37 chemical components were confirmed in negative ion mode,i.e.23 from Ginseng Radix Rhizoma and 14 from Astragali Radix.The proposed method is comprehensive and rapid in the identification of chemical components in Bufei Yishen formula,which provides the basic data to reveal its pharmacodynamics substances.