低强度激光对运动性免疫抑制大鼠肺部炎性反应进程的影响

Effects of low-power laser on the process of lung inflammation reaction of rats with exercise-induced immunosuppression

探讨低强度激光对运动性免疫抑制导致的肺组织炎症的调理作用。将8周龄雄性SD大鼠104只随机分为安静对照组、运动训练组、小剂量低强度激光^+运动组、大剂量低强度激光^+运动组。运动各组进行跑台训练,每次30min,每天1次,每周6d,持续6周。采用递增负荷强度(跑台速度第1周10 m/min、第2周20 m/min,从第3周开始,每周递增5 m/min,至第6周达到40 m/min)。激光干预采用氦氖激光鼻腔外照射(训练1次/d,2 min/次)。辐射功率小剂量组为1 mW (6.8 J/cm2),大剂量组2 mW (13.6 J/cm2)。分别于训练前及训练的第2、4、6周周末取材,检测肺灌洗液中sIgA、CRP、IL-4、IL-10、TNF-α、ICAM-1含量;分析肺组织中CD4^+和CD8^+阳性蛋白表达,并对肺脏进行组织形态学观察。结果发现:1)6周递增负荷训练期间,大鼠肺脏中促炎因子(TNF-α和ICAM-1)显著上升,抗炎因子(IL-4和IL-10)及sIgA、CD4^+与CD8^+比值显著下降。肺组织局部出血、脓液渗出、炎性细胞浸润逐渐加重。2)小剂量低强度激光照射可显著延缓运动大鼠肺组织上述变化,对抗炎因子和形态学的影响尤为显著;而大剂量低强度激光的干预效果随照射疗程的延长呈下降趋势。结果说明:小剂量的低强度激光鼻部照射可显著促进6周递增负荷训练大鼠的免疫机能和肺组织炎症反应的改善,其调节过程主要是通过上调抗炎因子的合成,促使"促炎-抗炎"细胞因子平衡。

In order to probe into low-power laser’s function on regulating lung tissue inflammation caused by exercise-induced immunosuppression,the authors divided 104 8-week old male SD rats randomly into a calm control group,an exercise training group,a small-dose low-power laser+exercise group,and a big-dose low-power laser+exercise group.The rats in the exercise groups carried out treadmill training for 6 weeks continuously,30min/time,1 time/day,6 days/week.The authors used incremental load intensities(treadmill speed:10m/min in week 1,20m/min in week 2,then increased incrementally by 5m/min per week,starting from week 3,to 40m/min in week 6).Laser intervention used was He-Ne laser nasal external irradiation(1 time/day on training days,2min/time).The irradiation power was 1mW(6.8 J/cm^2)for the small-dose group,2mW(13.6 J/cm^2)for the big-dose group.The authors took samples respectively before training and at the ends of weeks 2,4 and 6,measured the contents of sIgA,CRP,IL-4,IL-10,TNF-αand ICAM-1 in the bronchoalveolar lavage fluid,analyzed the expressions of positive proteins CD4^+and CD8^+in the lung tissues,and carried out histomorphological observation on the lungs,and revealed the following findings:1)during the 6-week incremental load training period,in the rats’lungs,pro-inflammation cytokines TNF-αand ICAM-1 increased significantly,anti-inflammation cytokines IL-4 and IL-10 as well as sIgA and the ratio of CD4^+to CD8^+decreased significantly;the lung tissues showed local breeding,pus exudation,and gradually worsening inflammatory cell infiltration;2)small-dose low-power laser irradiation can significantly slow the said changes of the exercise rats’lung tissues,its effects on anti-inflammation cytokines and morphology were especially significant;while the effect of big-dose low-power laser intervention showed a trend of decreasing as the irradiation process extended.The said findings indicate the followings:small-dose low-power laser nasal irradiation can significantly promote the improvement of immune function and lung tissue inflammation reaction of the rats carrying out 6-week incremental load training,its regulating process is mainly to promote the balance between pro-inflammation cytokines and anti-inflammation cytokines by up-regulating the synthesis of anti-inflammation cytokines.