托里消毒散治疗正虚邪恋型体表瘘管大鼠的实验研究

Experimental research of tuoli xiaodu powder in the treatment of subcutaneous fistula of the mixture of antipathogenic qi deficiency and retention of pathogenic factors in the model rats

目的通过建立正虚邪恋型体表瘘管大鼠模型,采用托里消毒散对其进行干预,探讨托里消毒散对模型大鼠的治疗作用。方法 32只SD大鼠分为正常组、模型组、生理盐水组(NS组)和补托组,采用腹腔注射环磷酰胺联合金黄色葡萄球菌和大肠杆菌混合菌液注入埋置于大鼠背部皮下的弹簧纱条的方法建立模型; NS组和补托组分别用生理盐水和托里消毒散进行灌胃治疗。对各组大鼠造模前后以及药物干预期间四诊计量化指标、NS组和补托组体表瘘管愈合时间进行比较。结果 d41成模时,模型组背部瘘管病理与临床瘘管表现相符;模型组、NS组和补托组气虚、血虚、阴虚以及阳虚程度均明显低于正常组,差异有统计学意义(P <0. 01);治疗后,正常组、NS组与补托组在d49、d56以及体表瘘管愈合时,三组气虚、血虚、阴虚和阳虚程度组间比较差异有统计学意义(P <0. 05,P <0. 01),补托组气虚、血虚和阳虚程度随时间变化上升情况优于NS组;补托组瘘管愈合天数少于NS组(P <0. 05)。结论采用腹腔注射环磷酰胺联合金黄色葡萄球菌和大肠杆菌混合菌液注入弹簧纱条的方法,能制备稳定的、可重复的正虚邪恋型体表瘘管大鼠模型。托里消毒散可改善模型大鼠的虚证程度,促进瘘管愈合。

Objective To explore the therapeutic effects of tuoli xiaodu powder in the rat modeled as subcutaneous fistula of the mixture of antipathogenic qi deficiency and retention of pathogenic factors. Methods a total of 32 SD rats were divided into a normal group,a model group,a normal saline group(NS group) and a tuoli xiaodu powder group(herbal power group). The intraperitoneal injection with cyclophosphamide combined with the mixed bacteria culture of staphylococcus aureus and escherichia coli was adopted. They were injected in the spring gauze subcutaneously embedded on the dorsum of rat in the modeling. In the NS group and the herbal powder group,the gavage treatment was given respectively with normal saline and tuoli xiaodu powder. Before and after treatment,as well as during the intervention,the quantitative indexes of four diagnostic methods were observed in each group. The fistula healing time was compared between the NS group and the herbal powder group. Results On day 41 of modeling,in the model group,the pathological condition of fistula on the dorsum of rat was conformed to the clinical fistula manifestation. In the model group,the NS group and the herbal powder group,the severity of qi deficiency,blood deficiency,yin deficiency and yang deficiency was less obviously than the normal group,indicating the significant differences(P < 0. 01). After treatment,in the normal group,the NS group and the herbal powder group,on day 49,day 56 as well as the time of subcutaneous fistula,the differences were significant in the severity of qi deficiency,blood deficiency and yang deficiency in comparison among the three groups(P < 0. 05,P <0. 01). In the herbal powder group,the severity of qi deficiency,blood deficiency and yang deficiency was reduced more obviously with the time passed by as compared with the NS group. The fistula healing days in the herbal powder group were less than the NS group(P < 0. 05). Conclusion The intraperitoneal injection with cyclophosphamide combined with the mixed bacteria culture of staphylococcus aureus and escherichia coli injected in the spring gauze is applicable for the stable,repeatable preparation of the rat model of subcutaneous fistula of the mixture of antipathogenic qi deficiency and retention of pathogenic factors. Tuoli xiaodu powder relieves the severity of deficiency syndrome and promotes fistula healing.