糖蜜对人工培养银叶真藓生长发育的影响

Influence of Molasses on the Growth of Artificial Cultured Bryum argenteum

为促进藓结皮人工扩繁,以世界广布种银叶真藓(Bryum argenteum)为对象,通过室内试验研究了不同浓度(3、6、10、20g·L^(-1)和30g·L^(-1))糖蜜溶液对银叶真藓生长发育的影响。结果表明,添加糖蜜溶液会降低银叶真藓萌发数量,其中添加10g·L^(-1)和20g·L^(-1)糖蜜溶液时银叶真藓萌发数量与对照组差异显著,分别为对照组的38%和29%。添加糖蜜溶液会影响银叶真藓生长速率,其中添加3g·L^(-1)糖蜜溶液可显著增加银叶真藓生长速率,盖度每周可增加11.56%,密度每周可增加6.67株·cm^(-2),而添加30g·L^(-1)糖蜜溶液时银叶真藓生长速率显著低于对照组。添加10、20g·L^(-1)和30g·L^(-1)糖蜜溶液能在培养末期维持银叶真藓的盖度和密度,而添加3g·L^(-1)和6g·L^(-1)糖蜜溶液在培养末期时密度会下降,降幅分别为最大密度的19%和15%。培养初期使用3g·L^(-1)糖蜜溶液而培养末期增大浓度到10g·L^(-1),可显著增加银叶真藓的盖度、密度,在培养末期可维持72.83%的盖度和41.25株·cm^(-2)的密度。糖蜜溶液作为葡萄糖的替代物能促进银叶真藓的生长发育,在大面积培养人工藓结皮时可推广使用。

This study explored the effect of molasses with different concentrations (3,6,10,20 and 30 g·L^-1 ) on the growth and development of Bryum argenteum which was a cosmopolitan moss species by the laboratory cultivation method.The results showed that adding molasses solution could reduce the germination of B.argenteum .The germination numbers of adding 10 and 20 g·L^-1 molasses solution were significantly different from the control,approximately 38% and 29% of the control,respectively.The growth rate of B.argenteum was also affected by molasses solution.The concentration of 3 g·L^-1 molasses solution significantly increased the growth rate of B.argenteum,by which average growth rate of coverage reached 11.56% per week,the density of the average growth rate reached 6.67 plants/cm 2 per week.However the 30 g·L^-1 molasses solution significantly reduce the growth rate compared to the control.Adding 10,20 and 30 g·L^-1 molasses solution could maintain the coverage and the density of B.argenteum at the end of the culture.The density of B.argenteum was reduced at the end of the cultivation period by 19% and 15% of maximum density under the treatment of 3 and 6 g·L^-1 molasses solution.The coverage and density of B.argenteum significantly increased when the concentration of molasses solution enhanced from 3 in the early stage of culture to 10 g·L^-1 at the end of cultivation,which also could maintain a coverage of 72.83% and a density of 41.25 plants/cm^2 at the end of culture.It was concluded that the molasses solution could be used as a substitute for glucose to promote the growth and development of B.argenteum in large-scale artificial cultivation.