当归补血汤及其拆方对骨髓抑制模型小鼠Wnt蛋白及其受体的影响

Effect of Danggui Buxue Decoction and It's Separate Herb on Wnt and Wnt's Receptor in Mice with Myelosuppression

目的探讨当归补血汤及其拆方对骨髓抑制模型小鼠Wnt蛋白及其受体的影响。方法应用环磷酰胺所致的骨髓抑制小鼠模型,全自动血细胞分析仪检测外周血白细胞、红细胞、血小板数,ELISA法检测骨髓Wnt3a蛋白,qPCR法检测骨髓造血干细胞Frizzled 2 mRNA表达水平。结果与正常组比较,模型组外周血白细胞、红细胞、血小板计数明显降低(P<0.05或P<0.01),骨髓Wnt3a蛋白、骨髓造血干细胞Frizzled 2 mRNA表达水平显著降低(P<0.01);与模型组比较,当归补血汤组外周血红细胞、血小板数明显升高(P<0.05或P<0.01),骨髓Wnt3a蛋白、骨髓造血干细胞Frizzled 2 mRNA表达水平明显提高(P<0.05或P<0.01);黄芪组、当归组上述指标与模型组比较,差异均无显著性意义(P>0.05)。结论①当归补血汤具有提高骨髓Wnt3a蛋白及骨髓造血干细胞Frizzled 2 mRNA表达水平的作用;②当归补血汤及其拆方对Wnt3a及其受体Frizzled 2 mRNA的调控作用,体现了中医气血相生理论。

Objective To investigate the effect of Danggui Buxue Decocton and it’s separate herb on Wnt and Wnt’s receptor in mice with myelosuppression.Methods The mice model of Myelosuppression was established by Cyclophosphamide(CTX),Automatic Blood Cell Analyzer tested the Peripheral Blood cell number,ELISA tested Wnt3a protein in Bone Marrow,qPCR tested Frizzled 2 mRNA expression in Bone Marrow Hematopoietic Stem Cells.Results Compared to the normal group,the number of WBC,RBC and PLT were decreased significantly in model group(P<0.05 or P<0.01);The value of Wnt3a protein and Frizzled 2 mRNA were decreased significantly(P<0.01);Compared to the model group,the number of RBC and PLT were increased significantly in Danggui Buxue Decoction group(P<0.05 or P<0.01);The value of Wnt3a protein and Frizzled 2 mRNA were increased significantly(P<0.05 or P<0.01),but the Huangqi group and the Danggui group had no significantly different(P>0.05).Conclusion First,Danggui Buxue Decoction can increased the value of Wnt3a protein and Frizzled 2 mRNA in Bone Marrow.Second,the regulation of Wnt3a and it’s receptor Frizzled 2 mRNA with Danggui Buxue Decoction and it’s separate herb,showed the correct of“Qi and Blood generate each other”in Traditional Chinese Medicine.