注射用三七素对动物实验性损伤的止血作用

The Hemostatic Effects of the Dencichine Injection on Experimental Injuries of Different Animals

目的观察注射用三七素止血促凝的作用。方法注射用三七素设为高、中、低三个剂量,分别通过家兔肝急性损伤性出血,小鼠试验性胃黏膜损伤以及大鼠肠系膜动脉出血等试验观察其经静脉给予实验动物后的止血效果;以及通过小鼠腹腔毛细血管通透性试验检测注射用三七素对血管通透性的影响。另于给药后0、30和60 min分别抽取家兔血液,经处理后检测凝血四项指标(APTT、PT、TT和Fbg)及用Elisa方法测定纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制物-1(PAI-1)含量。结果注射用三七素各剂量均可减少家兔急性肝损伤的出血时间和出血量,以及大鼠肠系膜动脉出血时间,并明显降低醋酸所引起的小鼠毛细血管通透性升高,其中以高剂量(家兔1.00 mg·kg^(-1),大鼠2.00 mg·kg^(-1),小鼠2.5 mg·kg^(-1))组效果最为显著(P<0.05)。此外,注射用三七素高、中、低三个剂量均可显著减少小鼠试验性胃黏膜损伤指数(P<0.01)。此外,高剂量的三七素具有缩短家兔凝血酶原时间的趋势及减低家兔血中纤溶酶源激活物抑制物-1含量随时间推移而迅速下降的作用。结论注射用三七素可明显缩短多种动物模型的出血时间,促进血液凝固,并降低模型动物毛细血管通透性。同时通过促进血液凝结和抑制纤溶发挥而止血作用。

Objective The aim of the study was to observe the hemostatic and procoagulant effect of the Dencichine Injection on different experimental animal models.Methods Dencichine Injection was administered intravenously to 3 groups of animals of each species respectively with certain experimental injuries at dose levels of low,medium and high.Results The bleeding time and content of acute injured liver in rabbits were obviously reduced by Dencichine Injection treatment at a dosage of 1.00 mg/kg,as well as the bleeding time of mesenteric arteries cut in rats at 2.00 mg/kg.Also,the increased vascular permeability of capillaries in mice were decreased by administration of Dencichine Injection 2.5 mg/kg.In addition,the mice stomach injury index were significantly reduced in all 3 dosage of Dencichine Injection treated groups.Furthermore,with the treatment of 1 mg/kg Dencichine in rabbits,the prothrombin time(PT)was tend to be shortening,and the decrease rate of plasminogen activator inhibitor-1(PAI-1)were obviously slow down.Conclusion Intravenous administration with Dencichine Injection in multiple models on different animal species with experimental injury caused bleeding could be reduced significantly via promoting coagulation and inhibiting fibrinolysis.