白藜芦醇保护缺血性脑卒中大鼠神经功能的作用机制研究

Protective Effect Mechanism of Resveratrol on Neurological Function in Rats with Ischemic Stroke

目的探讨白藜芦醇对缺血性脑卒中大鼠神经功能保护作用机制。方法选择清洁级健康雄性SD大鼠75只,将其分为对照组、模型组、白藜芦醇低剂量(10 mg/kg)、白藜芦醇中剂量(20 mg/kg)以及白藜芦醇高剂量组(30 mg/kg),造模后比较各组大鼠神经功能障碍评分、脑梗死体积、脑组织含水量、脑组织p53及Notch1蛋白表达情况。结果白藜芦醇可显著降低缺血性脑卒中大鼠神经功能障碍评分(P<0.05),随着白藜芦醇浓度的升高大鼠神经功能障碍评分明显降低(P<0.05)。各手术组大鼠脑梗死体积及脑组织含水量较假手术组明显升高(P<0.05),而白藜芦醇各组大鼠脑梗死体积及脑组织含水量较模型组明显降低(P<0.05),随着白藜芦醇浓度的升高,大鼠脑梗死体积及脑组织含水量明显降低(P<0.05)。各手术组大鼠脑组织p53及Notch1蛋白表达量均较假手术组明显升高(P<0.05),白藜芦醇大鼠脑组织p53及Notch1蛋白表达量较模型组明显降低(P<0.05),且白藜芦醇浓度升高大鼠脑组织p53及Notch1蛋白表达量明显降低(P<0.05)。结论白藜芦醇对缺血性脑卒中大鼠具有着显著的神经功能保护作用,可有效改善大鼠脑梗死面积及脑水肿程度,其神经保护作用机制可能与白藜芦醇对p53介导的凋亡通路及Notch1介导的炎症反应通路抑制作用有关。

Objective To investigate the neuroprotective effect of resveratrol on ischemic stroke in rats and its mechanism.Methods 75 healthy male SD rats of clean grade were divided into control group,model group,low dose of resveratrol(10 mg/kg),middle dose of resveratrol(20 mg/kg)and high dose of resveratrol(30 mg/kg).The neurological impairment score,cerebral infarction volume,water content of brain tissue were compared after modeling.The expression of p53 and Notch1 protein in brain tissue.Results Resveratrol significantly decreased the neurological impairment score of ischemic stroke rats(P<0.05),and decreased the neurological impairment score with the increase of resveratrol concentration(P<0.05).The volume of cerebral infarction and water content of brain tissue in each operation group were significantly higher than those in sham operation group(P<0.05),while the volume of cerebral infarction and water content of brain tissue in resveratrol groups were significantly lower than those in model group(P<0.05),and the volume of cerebral infarction and water content of brain tissue were significantly decreased with the increase of resveratrol concentration(P<0.05).The expression of p53 and Notch1 protein in brain tissue of rats in each operation group was significantly higher than that in sham operation group(P<0.05),and the expression of p53 and Notch1 protein in resveratrol group was significantly lower than that in model group(P<0.05),and the expression of p53 and Notch1 protein in brain tissue of rats with resveratrol concentration increased significantly(P<0.05).Conclusion Resveratrol has significant neuroprotective effect on ischemic stroke rats,and can effectively improve the area of cerebral infarction and the degree of cerebral edema in rats.The neuroprotective mechanism may be related to the inhibition of resveratrol on p53-mediated apoptosis pathway and Notch1-mediated inflammatory response pathway.