摘要
目的:探讨凋亡蛋白酶活化因子1(Apaf-1)在甲状腺乳头状癌(PTC)中的m RNA与蛋白表达及其与细胞增殖的关系。方法:分别采用real-time PCR、Western blot及免疫组化法检测并比较甲状腺乳头状癌和癌旁组织中Apaf-1的m RNA及蛋白表达情况,并分析其表达水平与PTC临床病理学特征的关系;通过下调CGTHW-3细胞中Apaf-1表达量,验证Apaf-1对细胞增殖的影响。结果:在PTC组织中,Apaf-1的m RNA和蛋白表达量均显著低于癌旁组织(P <0. 05);下调Apaf-1表达增强了CGTHW-3细胞的增殖活性(P <0. 05)。结论:Apaf-1在PTC中低表达,抑制其表达可增强CGTHW-3细胞的增殖能力。Apaf-1在甲状腺乳头状癌中可能发挥抑癌作用。
AIM:To investigate the mRNA and protein expression of apoptotic protease-activating factor 1(Apaf-1)in papillary thyroid carcinoma(PTC)and its relationship with cell proliferation.METHODS:The methods of real-time PCR,Western blot and immunohistochemistry were used to detect the mRNA and protein expression of Apaf-1 in PTC and tumor-adjacent tissues.The relationship between Apaf-1 expression and clinicopathological characteristics was analyzed.The effect of Apaf-1 on cell proliferation was verified by down-regulating the expression of Apaf-1 in CGTHW-3 cells.RESULTS:The mRNA and protein expression levels of Apaf-1 in the PTC tissue were significantly lower than those in the tumor-adjacent tissue(P<0.05).Down-regulation of Apaf-1 expression enhanced the proliferation of CGTHW-3 cells(P<0.05).CONCLUSION:The expression of Apaf-1 is low in PTC,and inhibition of its expression enhances the proliferation of CGTHW-3 cells.Apaf-1 may play a tumor suppressor role in PTC.
作者
李益波
陈冠桦
戴岳楚
伍慧慧
麻怀露
俞盛健
梁勇
LI Yi-bo;CHEN Guan-hua;DAI Yue-chu;WU Hui-hui;MA Huai-lu;YU Sheng-jian;LIANG Yong(Hebei North University,Zhangjiakou 075000,China;Taizhou University,Taizhou 318000,China;The First Affiliated Hospital,Wenzhou Medical University,Wenzhou 325000,China;The Affiliated Hospital,Taizhou University,Taizhou 318000,China)
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2019年第1期55-61,共7页
Chinese Journal of Pathophysiology
基金
浙江省自然科学基金资助项目(No.LY14H160036)
台州市科技计划项目(No.1501KY31)
关键词
凋亡蛋白酶活化因子1
甲状腺乳头状癌
细胞增殖
Apoptotic protease-activating factor 1
Papillary thyroid carcinoma
Cell proliferation
