血管生成抑制蛋白1与肿瘤血管及内镜下的表现关系分析

Analysis of the relationship between the expression of angiogenesis inhibitor 1 in tumor vessel and endoscopic manifestations

目的:探讨血管生成抑制蛋白1(VASH1)在食管癌肿瘤血管中的表达与内镜下表现的关系。方法:选取存档石蜡包埋食管癌组织标本120例和正常食管组织标本50例,免疫组织化学SP法检测其VASH1的表达,分析VASH1在食管癌组织和正常食管组织中的形态差异,分析不同IPCL分型VASH1表达情况,以及不同肿瘤分化状态下VASH1表达与MVD的关系。结果:VASH1、血管内皮生长因子(VEGF)在食管癌组织中的阳性表达率分别为54. 17%(65/120)、59. 17%(71/120),在正常组织中的阳性表达率分别为8. 0%(4/50)、16. 0%(8/50),VASH1、VEGF在食管癌组织中的阳性表达率高于正常组织(P <0. 01);食管癌组织中,VEGF与VASH1表达呈显著正相关关系,影响食管癌预后效果的独立危险因素有浸润深度、淋巴结转移、VASH1及VEGF表达水平等(P <0. 05～P <0. 01);上皮乳头内毛细血管襻Ⅴ型病人占比最高为54. 79%,Ⅳ型为56. 82%,差异无统计学意义(P> 0. 05); VASH1阴性、低表达、高表达病人的MVD值差异有统计学意义(P <0. 01),随着VASH1表达增加,病人MVD值增高,VASH1表达与血管形成呈正相关关系(P <0. 01)。结论:VASH1可能与食管癌的发展、肿瘤细胞生成密切相关,且对预测食管癌的预后具有一定的意义。

Objective: To investigate the relationship between the expression of angiogenesis inhibitor 1( VASH1) and endoscopic manifestations in esophageal carcinoma. Methods: The expression levels of VASH1 in 120 paraffin embedded esophageal carcinoma samples and 50 normal esophageal tissues samples were detected using immunohistochemical SP method. The expression difference of VASH1 between esophageal carcinoma and normal esophageal tissue was analyzed. The expression levels of VASH1 in different IPCL types,and relationship between VASH1 expression and MVD in different differentiation tumors were analyzed. Results: The positive expression rates of VASH1 and VEGF in esophageal carcinoma were 54. 17%( 65/120) and 59. 17%( 71/120),respectively,and the positive expression rates of VASH1 and VEGF in normal VASH1 tissue were 8. 0%( 4/50) and 16. 0%( 8/50),respectively. The positive rates of VASH1 and VEGF in esophageal carcinoma were significantly higher than those in normal esophageal tissue( P <0. 01). The expression level of VEGF was positively correlated with the expression of VASH1 in esophageal carcinoma,and the expression levels of VEGF and VASH1,depth of invasion and lymph node metastasis were the independent risk factors in influencing the prognosis of esophageal carcinoma( P < 0. 05 to P < 0. 01). The percentages of IPCL-Ⅴ and IPCL-Ⅳ were 54. 79% and 56. 82%,respectively,and the difference of which was not statistically significant( P > 0. 05). The differences of MVD value among the patients with negative,low and high VASH1 expressions were statistically significant( P < 0. 01). With the increasing of VASH1 expression level of patients,the MVD value increased,and the VASH1 expression was positively correlated with microvessel density( P < 0. 01).Conclusions: VASH1 may be closely related to the development of esophageal carcinoma and tumor cell formation,which has certain significance in predicting the prognosis of esophageal carcinoma.