结直肠癌发病相关关键lncRNA的筛选和鉴定:基于肿瘤基因图谱数据库的生物信息学分析

Screening and identification of key lncRNA associate with carcinogenesis and development of CRC: a bioinformatic analysis of TCGA

目的探讨长链非编码RNA(lncRNA)在结直肠癌(CRC)中的表达特征及其对患者生存预后判断的潜在价值。方法下载肿瘤基因图谱(TCGA)中CRC相关的RNA序列(RNA-seq)数据,进行lncRNA表达差异、功能、通路富集、调控网络及lncRNA-mRNA共表达分析,同时分析lncRNA差异表达对CRC患者预后的影响。结果对TCGA 453个CRC临床样本数据进行分析,提示有119个lncRNA存在差异性表达,lncRNA和编码基因存在共表达关系,对基因富集性分析发现,lncRNA参与细胞的多个生物学行为;16个lncRNA对患者生存预后显著相关,其中3个lncRNA BVES-AS1、HAND2-AS1和MAMDC2-AS1为关键lncRNA,可作为预后判断的标志物,同时与mi RNA、coding gene存在紧密的调控网络。结论 lncRNA在CRC中存在差异性表达,起着重要的调控作用,在CRC患者诊断、靶向治疗及预后判断方面具有潜在的应用价值。

Objective To expore the lncRNA expression profiles and features in CRC tissues and their potential value associated with prognosis assessment.Methods The differential expression,functional and pathway enrichment,interaction network and lncRNA-mRNA co-expression of lncRNA wereanalyzed using RNA-seq data from TCGA.The prognosis changes of CRC patients induced by the differential expression level of lncRNA wereanalyzed.Results TCGA RNA-seq data from 453 specimens of CRC patients showed that there were 119 lncRNA which expressed differentially.lncRNA and coding gene had the relation of co-expression.lncRNA played various roles in biological features of cells.There existed lncRNA-miRNA-mRNA regulatory network.16 of these lncRNA had potential value on the prognosis of CRC patients,3 of them were key lncRNA,which were BVES-AS1,HAND2-AS1 and MAMDC2-AS1.They could be seemed as the biomarkers for prognosis of CRC patients.They also had close relations with the miRNA and coding gene.Conclusion lncRNA express differently in CRC tissues,and play an important role in biological feature of CRC cells,which have potential value in diagnosis,targeted therapy and prognosis of CRC patients.