灯盏乙素对乳腺癌细胞中lncRNAs表达的影响

Impacts of scutellarin on the expression of LncRNAs in breast cancer cells

检测灯盏乙素对乳腺癌MDA-MB-231细胞中的长链非编码RNA(LncRNAs)的相对表达量的影响,进一步探索灯盏乙素对肿瘤的作用机制.采用实时荧光定量PCR的方法,检测对照组与不同剂量组的灯盏乙素处理后的乳腺肿瘤细胞中lncRNAs MALAT1、NEAT1和p53基因mRNA的相对表达量,并观察细胞存活率.结果发现,灯盏乙素在低剂量(1、10、25μmol/L)时促进细胞增殖,而在高剂量50μmol/L以上时抑制细胞增殖.灯盏乙素在低剂量时使得乳腺癌MDA-MB-231细胞中MALAT1,NEAT1和p53基因的表达水平下降,而在100μmol/L的高剂量时MALAT1,NEAT1和p53基因的表达水平上升.灯盏乙素影响乳腺癌MDA-MB-231细胞中LncRNAs MALAT1和NEAT1基因以及p53基因的表达,并且存在剂量反应关系,低剂量与高剂量呈现出相反的表达量变化.

This research aimed to investigate the effect of scutellarin on the relative expression of long non-coding RNAs (LncRNAs) in breast cancer MDA-MB-231 cells, and explored the inhibition mechanism of scutellarin for tumors. MDA-MB-231 breast cancer cells were treated with scutellarin (1 μmol/L, 10 μmol/L, 25 μmol/L, 50 μmol/L and 100 μmol/L) for 5 days. The relative expression of LncRNAsMALAT1 and NEAT1and mRNA expressions of p53 gene in breast cancer cells was detected by the real-time quantitative PCR. The inhibition of cell growth was assessed. It was concluded that scutellarin promoted the cell proliferation at the low concentrations (1, 10, 25 μmol/L)but conversely inhibited at the concentrations above 50 μmol/L. Similarly, the gene expression levels of MALAT1, NEAT1 and p53 gene were down-regulated at low doses but significantly up-regulated at the high dose of 100 μmol/L. Thus, scutellarin has an effect on the expression of LncRNAs MALAT1 and NEAT1with a dose-response relationship, while the low dose and the high dose showed opposite expression changes.