miRNA-1271对人乳腺癌细胞增殖的影响

Effects of mi RNA-1271 on proliferation of human breast cancer cells

目的探讨miRNA-1271对人乳腺癌细胞增殖的影响。方法采用RT-PCR法检测80例乳腺癌组织及癌旁正常组织中miRNA-1271的表达。采用脂质体转染法将miRNA-1271模拟物转染至乳腺癌细胞株MCF-7和MDA-MB-231中,应用CCK-8实验、克隆形成实验、流式细胞术、动物实验等探究miRNA-1271对乳腺癌细胞增殖、凋亡的影响。结果乳腺癌组织中miRNA-1271相对表达量较癌旁正常组织明显降低(P<0.05)。miRNA-1271表达上调后,MCF-7和MDA-MB-231乳腺癌细胞的吸光度值明显降低(均P<0.05),克隆形成数明显减少(均P<0.05),细胞凋亡率明显增高(均P<0.05)。动物实验结果显示,miRNA-1271在体内亦能抑制MCF-7乳腺癌细胞的增殖。结论 miRNA-1271在乳腺癌组织中低表达;上调miRNA-1271表达,能抑制乳腺癌细胞的增殖,促进凋亡的发生,具有抑癌作用。

Objective To investigate the effect of mi RNA-1271 on proliferation of human breast cancer cells. Methods The expression levels of mi RNA-1271 were detected with RT-PCR in 80 samples of breast cancer and pericancerous breast tissues. Human breast cancer MCF-7 and MDA-MB-231 cells were transfected with mi RNA-1271 mimics, the proliferation of breast cancer cells was determined by CCK-8 and colony formation assays, cell apoptosis was examined by flow cytometry. The mi RNA-1271-transfected breast cancer MCF-7 cells were inoculated in nude mice and the growth of transplanted tumors was measured. Results The expression level of mi RNA-1271 in breast cancer tissues were significantly lower than that in pericancerous breast tissues(P <0.05). CCK-8 assay showed that the OD490 value was significantly lower in mi RNA-1271-expressing breast cancer cells(P<0.05). The number of clone formation cells was lower and the apoptosis rate was significantly higher than those in control group(P<0.05). Animal experiments showed that mi RNA-1271 also inhibited the growth of MCF-7 breast cancer in vivo.Conclusion mi RNA-1271 is down-regulated in breast cancer tissues. Up-regulated mi RNA-1271 significantly inhibits the proliferation of breast cancer cells and increases cell apoptosis.