甲状腺乳头状癌中FMNL2和EMT相关蛋白的表达及其临床意义

Expression of FMNL2 and EMT-related proteins in papillary thyroid carcinoma and its clinical significance

目的探讨同源形成素样蛋白2(FMNL2)和上皮间质转化(EMT)相关蛋白E-钙粘素、波形蛋白在甲状腺乳头状癌(PTC)中联合表达的临床意义。方法采用免疫组化En Vision法检测80例PTC和30例相应癌旁组织中FMNL2、E-钙粘素和波形蛋白的表达差异,并分析PTC组织FMNL2、E-钙粘素和波形蛋白之间表达及与临床病理特征及指标相互之间的关系。结果FMNL2、波形蛋白在PTC组织中阳性表达率分别为61.25%(49/80)和76.25%(61/80),癌旁组织分别为13.33%(4/30)和20.00%(6/30),差异均有统计学意义(均P<0.05),而E-钙粘素在PTC组织中的阳性表达率为32.50%(26/80),显著低于癌旁组织的80.00%(24/30),差异有统计学意义(P<0.05)。包膜有无侵犯、淋巴结是否转移及不同临床分期者间FMNL2、E-钙粘素和波形蛋白表达差异均有统计学意义(均P<0.05)。淋巴结转移灶中FMNL2和波形蛋白表达均高于原发灶(P<0.05),而淋巴结转移灶中E-钙粘素表达低于原发灶(P<0.05)。PTC组织中FMNL2表达与E-钙粘素表达呈负相关(r=-0.325,P<0.05),FMNL2表达与波形蛋白表达呈正相关(r=0.340,P<0.05)。结论 PTC组织中FMNL2、波形蛋白阳性表达明显升高,而E-钙粘素表达下调,FMNL2表达与EMT相关蛋白表达密切相关,提示3者异常表达可能联合参与PTC的发生、发展过程。

Objective To investigate the clinical significance of expression of formin-like protein 2(FMNL2)and epithelial mesenchymal transformation(EMT)-related proteins(E-cadherin)and(Vimentin)in thyroid papillary carcinoma(PTC).Methods The expression of FMNL2,E-cadherin and Vimentin in 80 samples of PTC and 30 samples of corresponding adjacent tissues were detected by immunohistochemical En Vision method,and the correlations among the expression of FMNL2,E-cadherin and Vimentin in PTC and their relationship with the clinicopathological features were analyzed.Results The positive rates of FMNL2 and Vimentin in PTC tissues were 61.25%(49/80)and 76.25%(61/80),respectively,those in adjacent tissues were 13.33%(4/30)and 20.00%(6/30),there were significant differences between them(P<0.05);while the positive expression rate of E-cadherin in PTC tissue was 32.50%(26/80),which was significantly lower than that of adjacent tissue(80.00%,24/30,P<0.05).The expressions of FMNL2,E-cadherin and Vimentin were significantly correlated with the capsule invasion,lymph node metastasis and clinical stages(P<0.05).The expressions of FMNL2 and Vimentin in metastatic lymph nodes were higher than those in primary tumor(P<0.05),while the expression of E-cadherin in metastatic lymph nodes was lower than that in primary tumor(P<0.05).The expression of FMNL2 in PTC tissues was negatively correlated with the expression of E-cadherin(r=-0.325,P<0.05),and the expression of FMNL2 was positively correlated with the expression of Vimentin(r=0.340,P<0.05).Conclusion The expression of FMNL2 and Vimentin in PTC tissues is significantly up-regulated,but the expression of E-cadherin is down-regulated in PTC.The expression of FMNL2 is closely correlated with the expression of EMT-related proteins.Abnormal expression of FMNL2 and EMT-related proteins may be involved in the occurrence and progression of PTC.